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. 1999 Mar;37(3):788-91.
doi: 10.1128/JCM.37.3.788-791.1999.

The IS6110 restriction fragment length polymorphism in particular multidrug-resistant Mycobacterium tuberculosis strains may evolve too fast for reliable use in outbreak investigation

A Alito  1 , N Morcillo, S Scipioni, A Dolmann, M I Romano, A Cataldi, D van Soolingen
Affiliations

The IS6110 restriction fragment length polymorphism in particular multidrug-resistant Mycobacterium tuberculosis strains may evolve too fast for reliable use in outbreak investigation

A Alito et al. J Clin Microbiol. 1999 Mar.

Abstract

To study possible nosocomial transmission of multidrug-resistant (MDR) Mycobacterium tuberculosis, strain types and other information on 24, mostly human immunodeficiency virus-positive patients, were collected. Isolates from 11 patients had identical IS6110 restriction fragment length polymorphism (RFLP) patterns as well as spoligotype patterns and resistance profiles. Noticeably, nine other isolates from related cases also exhibited identical spoligotypes but slightly different RFLP patterns. These results indicate that for some MDR strains, the evolutionary clock of IS6110 RFLP may run too fast for reliable interpretation of strain typing results over a period of a few years.

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Figures

FIG. 1
FIG. 1
Patient information, drug resistance profiles, and DNA fingerprints of M. tuberculosis isolates related to the two described TB outbreaks. (A) First outbreak; (B) second outbreak. The column “Drug susc.” indicates resistance to the drugs abbreviated as follows: H, isoniazid; S, streptomycin; R, rifampin; E, ethambutol; Z, pyrazinamide. ND, not determined. Dates are given as month/year. ∗, period of patient’s attendance at hospital H1; ∗∗, period of patient’s attendance at hospital H2.
FIG. 2
FIG. 2
IS6110 RFLP patterns of two M. tuberculosis strains isolated from an HIV-negative patient (patient 23) at an interval of 1 year. The RFLP pattern of the first isolate, shown in lane A, contains one additional band at the 1.5-kb position. Both strains exhibited the unique spoligotype F.
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