Electronmicroscopic cytochemical studies on lysosomes and peroxisomes of a rat cardiac muscle in the experimental alloxan diabetes

Abstract

The combined electronmicroscopic and cytochemical investigations of lysosomes and peroxisomes of a rat cardiac muscle were carried out in various duration time of the alloxan diabetes. The analysis of cytochemical reactions with DAB, confirming the existence of peroxisomes in rat heart muscle revealed that the state of experimental alloxan diabetes did not at all affect either cytochemical or morphological image of peroxisomes. In comparison with the control group, in all 3 experimental groups cytochemical reaction to acid phosphatase markedly pointed to the increase in number and size of electron-dense bodies (lysosomes) with positive reaction. This state may most probably result both from local and general organism disturbances in metabolism, induced by particular experimental conditions.