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International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant.
Whale AS, Devonshire AS, Karlin-Neumann G, Regan J, Javier L, Cowen S, Fernandez-Gonzalez A, Jones GM, Redshaw N, Beck J, Berger AW, Combaret V, Dahl Kjersgaard N, Davis L, Fina F, Forshew T, Fredslund Andersen R, Galbiati S, González Hernández Á, Haynes CA, Janku F, Lacave R, Lee J, Mistry V, Pender A, Pradines A, Proudhon C, Saal LH, Stieglitz E, Ulrich B, Foy CA, Parkes H, Tzonev S, Huggett JF. Whale AS, et al. Among authors: huggett jf. Anal Chem. 2017 Feb 7;89(3):1724-1733. doi: 10.1021/acs.analchem.6b03980. Epub 2017 Jan 18. Anal Chem. 2017. PMID: 27935690
Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study.
Doyle RM, O'Sullivan DM, Aller SD, Bruchmann S, Clark T, Coello Pelegrin A, Cormican M, Diez Benavente E, Ellington MJ, McGrath E, Motro Y, Phuong Thuy Nguyen T, Phelan J, Shaw LP, Stabler RA, van Belkum A, van Dorp L, Woodford N, Moran-Gilad J, Huggett JF, Harris KA. Doyle RM, et al. Among authors: huggett jf. Microb Genom. 2020 Feb;6(2):e000335. doi: 10.1099/mgen.0.000335. Epub 2020 Feb 12. Microb Genom. 2020. PMID: 32048983 Free PMC article.
Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2.
Huggett JF, Benes V, Bustin SA, Garson JA, Harris K, Kammel M, Kubista M, McHugh TD, Moran-Gilad J, Nolan T, Pfaffl MW, Salit M, Shipley G, Vallone PM, Vandesompele J, Wittwer C, Zeichhardt H. Huggett JF, et al. Clin Chem. 2020 Nov 1;66(11):1369-1372. doi: 10.1093/clinchem/hvaa214. Clin Chem. 2020. PMID: 32894756 Free PMC article.
An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities.
O'Sullivan DM, Doyle RM, Temisak S, Redshaw N, Whale AS, Logan G, Huang J, Fischer N, Amos GCA, Preston MD, Marchesi JR, Wagner J, Parkhill J, Motro Y, Denise H, Finn RD, Harris KA, Kay GL, O'Grady J, Ransom-Jones E, Wu H, Laing E, Studholme DJ, Benavente ED, Phelan J, Clark TG, Moran-Gilad J, Huggett JF. O'Sullivan DM, et al. Among authors: huggett jf. Sci Rep. 2021 May 19;11(1):10590. doi: 10.1038/s41598-021-89881-2. Sci Rep. 2021. PMID: 34012005 Free PMC article.
Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics.
Whale AS, von der Heide EK, Kohlenberg M, Brinckmann A, Baedker S, Karalay O, Fernandez-Gonzalez A, Busby EJ, Bustin SA, Hauser H, Missel A, O'Sullivan DM, Huggett JF, Pfaffl MW, Nolan T. Whale AS, et al. Among authors: huggett jf. Methods. 2022 May;201:5-14. doi: 10.1016/j.ymeth.2021.08.006. Epub 2021 Aug 26. Methods. 2022. PMID: 34454016 Free PMC article.
The Dangers of Using Cq to Quantify Nucleic Acid in Biological Samples: A Lesson From COVID-19.
Evans D, Cowen S, Kammel M, O'Sullivan DM, Stewart G, Grunert HP, Moran-Gilad J, Verwilt J, In J, Vandesompele J, Harris K, Hong KH, Storey N, Hingley-Wilson S, Dühring U, Bae YK, Foy CA, Braybrook J, Zeichhardt H, Huggett JF. Evans D, et al. Among authors: huggett jf. Clin Chem. 2021 Dec 30;68(1):153-162. doi: 10.1093/clinchem/hvab219. Clin Chem. 2021. PMID: 34633030
135 results