Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 Jan 11;40(2):68.
doi: 10.1007/s11274-024-03885-4.

Progress in research on the biosynthesis of 1,2,4-butanetriol by engineered microbes

Xiangyu Ma  1   2 , Chao Sun  1   3   4 , Mo Xian  1   3   4 , Jing Guo  5   6   7 , Rubing Zhang  8   9   10
Affiliations
Review

Progress in research on the biosynthesis of 1,2,4-butanetriol by engineered microbes

Xiangyu Ma et al. World J Microbiol Biotechnol. .

Abstract

1,2,4-butanetriol (BT) is a polyol with unique chemical properties, which has a stereocenter and can be divided into D-BT (the S-enantiomer) and L-BT (the R-enantiomer). BT can be used for the synthesis of 1,2,4-butanetriol trinitrate, 3-hydroxytetrahydrofuran, polyurethane, and other chemicals. It is widely used in the military industry, medicine, tobacco, polymer. At present, the BT is mainly synthesized by chemical methods, which are accompanied by harsh reaction conditions, poor selectivity, many by-products, and environmental pollution. Therefore, BT biosynthesis methods with the advantages of mild reaction conditions and green sustainability have become a current research hotspot. In this paper, the research status of microbial synthesis of BT was summarized from the following three aspects: (1) the biosynthetic pathway establishment for BT from xylose; (2) metabolic engineering strategies employed for improving BT production from xylose; (3) other substrates for BT production. Finally, the challenges and prospects of biosynthetic BT were discussed for future methods to improve competitiveness for industrial production.

Keywords: 1,2,4-Butanetriol; Biosynthesis; Key enzymes; Renewable materials; Xylose.

PubMed Disclaimer

References

    1. Abdel-Ghany SE, Day I, Heuberger AL, Broeckling CD, Reddy AS (2013) Metabolic engineering of Arabidopsis for butanetriol production using bacterial genes. Metab Eng 20:109–120. https://doi.org/10.1016/j.ymben.2013.10.003
    1. Bamba T, Yukawa T, Guirimand G, Inokuma K, Sasaki K, Hasunuma T, Kondo A (2019) Production of 1,2,4-butanetriol from xylose by Saccharomyces cerevisiae through Fe metabolic engineering. Metab Eng 56:17–27. https://doi.org/10.1016/j.ymben.2019.08.012 - DOI - PubMed
    1. Bañares AB, Valdehuesa KNG, Ramos KRM, Nisola GM, Lee WK, Chung WJ (2019) Discovering a novel D-xylonate-responsive promoter: the PyjhI-driven genetic switch towards better 1,2,4-butanetriol production. Appl Microbiol Biotechnol 103(19):8063–8074. https://doi.org/10.1007/s00253-019-10073-0
    1. Bañares AB, Nisola GM, Valdehuesa KNG, Lee WK, Chung WJ (2021) Understanding D-xylonic acid accumulation: a cornerstone for better metabolic engineering approaches. Appl Microbiol Biotechnol 105(13):5309–5324. https://doi.org/10.1007/s00253-021-11410-y - DOI - PubMed
    1. Bera AK, Ho NWY, Khan A, Sedlak M (2011) A genetic overhaul of Saccharomyces cerevisiae 424A(LNH-ST) to improve xylose fermentation. J Ind Microbiol Biotechnol 38(5):617–626. https://doi.org/10.1007/s10295-010-0806-6 - DOI - PubMed

LinkOut - more resources