CRISPR/Cas9-mediated mutation of GhCAD decreases the gossypol content of cottonseed.
Zhou L, Wang Y, Wang J, Wang P, Lu G, Su X, Afridi M, Guo H, Cheng H.
Zhou L, et al.
J Biol Eng. 2025 Sep 30;19(1):83. doi: 10.1186/s13036-025-00556-2.
J Biol Eng. 2025.
PMID: 41029731
Free PMC article.
However, high free gossypol contents limit the application of cottonseed in the food or feed industry. In this study, CRISPR/Cas9 technology was used to knock out the (+)-delta-cadinene synthase gene (GhCAD) to decrease the gossypol content. ...Dual-luciferase assays revea …
However, high free gossypol contents limit the application of cottonseed in the food or feed industry. In this study, CRISPR/Cas9 technol …