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. 2002 Feb;184(3):861-3.
doi: 10.1128/JB.184.3.861-863.2002.

NADPH-dependent L-sorbose reductase is responsible for L-sorbose assimilation in Gluconobacter suboxydans IFO 3291

Masako Shinjoh  1 , Masaaki Tazoe, Tatsuo Hoshino
Affiliations

NADPH-dependent L-sorbose reductase is responsible for L-sorbose assimilation in Gluconobacter suboxydans IFO 3291

Masako Shinjoh et al. J Bacteriol. 2002 Feb.

Abstract

The NADPH-dependent L-sorbose reductase (SR) of L-sorbose-producing Gluconobacter suboxydans IFO 3291 contributes to intracellular L-sorbose assimilation. The gene disruptant showed no SR activity and did not assimilate the once-produced L-sorbose, indicating that the SR functions mainly as an L-sorbose-reducing enzyme in vivo and not as a D-sorbitol-oxidizing enzyme.

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Figures

FIG. 1.
FIG. 1.
The metabolic pathway of d-sorbitol, l-sorbose, and their metabolites in Gluconobacter strains. *1, membrane-bound d-sorbitol dehydrogenase (2); *2, membrane-bound l-sorbose dehydrogenase (2); *3, NAD(P)-dependent l-sorbosone dehydrogenase (2); *4, NADPH-dependent l-sorbosone reductase (2); *5, NADPH-dependent l-sorbose reductase (2,11); *6, NAD-dependent d-sorbitol dehydrogenase (1).
FIG. 2.
FIG. 2.
Computer analysis of the sequences of upstream and downstream regions of the SR gene. B, BamHI; EI, EcoRI; EO, EcoO1091; EV, EcoRV; H, HindIII; S, SmaI.
See this image and copyright information in PMC

References

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