Rotavirus G5P[6] in child with diarrhea, Vietnam

Abstract

We detected rotavirus G5P[6] with a long RNA pattern in a Vietnamese child with diarrhea. Viral outer capsid protein VP7 and VP4 genes suggest that it likely originated from porcine rotavirus either by genetic reassortment or as whole virions. To our knowledge, this is the first report of human rotavirus G5 in Asia.

Figure 1

A) Phylogenetic tree constructed from deduced amino acid sequences of the VP7 gene of animal and human G5 rotaviruses. Strain 4695G5, an equine G3 strain, was used as an outgroup. Bootstrap values are expressed as percentages. Bootstrap value <50 is not shown. Strain KH210 clustered with the human G5 rotavirus from Cameroon (MRC3105) and other G5 rotaviruses of porcine origin from Australia and Argentina. The Brazilian human isolate of G5 rotavirus clustered with G5 rotaviruses of porcine and equine origin. Species of origin followed by country of isolation is shown in parentheses after the strain name. B) Phylogenetic tree constructed from the deduced amino acid sequences of the VP8* gene of rotaviruses representing all P[6] lineages. Strain OSU was used as an outgroup. Bootstrap values are expressed as percentages. A bootstrap value <50 is not shown. Strains KH210 and KH228 clustered with lineage Ic. Species of origin followed by country of isolation is shown in parentheses after the strain name. Scale bar shows genetic distance expressed as amino acid substitutions per site.

Figure 2

Phylogenetic tree constructed from the deduced amino acid sequences of the NSP4 gene of rotaviruses representing all genotypes. Strain Bristol, a group C rotavirus, was used as an outgroup. Bootstrap values are expressed as percentages. Bootstrap value <50 is not shown. Strain KH210 clustered with strains in genotype B. Species of origin is shown in parentheses after the strain name. Scale bar shows genetic distance expressed as amino acid substitutions per site.