Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Feb;46(2):780-4.
doi: 10.1128/JCM.01615-07. Epub 2007 Dec 12.

Discrimination of herpes simplex virus type 2 strains by nucleotide sequence variations

Hisatoshi Kaneko  1 , Takashi Kawana, Ken Ishioka, Eiko Fukushima, Tatsuo Suzutani
Affiliations

Discrimination of herpes simplex virus type 2 strains by nucleotide sequence variations

Hisatoshi Kaneko et al. J Clin Microbiol. 2008 Feb.

Abstract

We determined the polymorphous 400-bp regions in UL53, US1, and US4 for the discrimination of herpes simplex virus type 2 (HSV-2) strains. Thirty-six HSV-2 clinical strains could be differentiated into 35 groups using these three regions and into 36 groups by additional analysis of three noncoding regions previously reported as polymorphous.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Nucleotide diversity of each HSV-2 gene. The diversities of 19 UL and 4 US genes were analyzed using sequence data registered in GenBank.
FIG. 2.
FIG. 2.
Phylogenetic analyses of 400-bp regions in the UL53 (A), US1 (B), and US4 (C) genes and in NC3 (D). Sequence data from all 36 HSV-2 clinical strains (shown as strains 1 to 36) and strain HG52 (3) were analyzed by the neighbor-joining method. The names of strains with common sequences in the 400-bp regions from UL53 and US4 are shown in red and underlined, respectively. The 26 American strains registered in GenBank (7) were added for the phylogenetic analysis of NC3. These American strains are shown in blue.
See this image and copyright information in PMC

References

    1. Barrett-Muir, W., F. T. Scott, P. Aaby, J. John, P. Matondo, Q. L. Chaudhry, M. Siqueira, A. Poulsen, K. Yamanishi, and J. Breuer. 2003. Genetic variation of varicella-zoster virus: evidence for geographical separation of strains. J. Med. Virol. 70(Suppl. 1)S42-S47. - PubMed
    1. Chiba, A., T. Suzutani, M. Saijo, S. Koyano, and M. Azuma. 1998. Analysis of nucleotide sequence variations in herpes simplex virus types 1 and 2, and varicella-zoster virus. Acta Virol. 42401-407. - PubMed
    1. Dolan, A., F. E. Jamieson, C. Cunningham, B. C. Barnett, and D. J. McGeoch. 1998. The genome sequence of herpes simplex virus type 2. J. Virol. 722010-2021. - PMC - PubMed
    1. Hammerberg, O., J. Watts, M. Chernesky, I. Luchsinger, and W. Rawls. 1983. An outbreak of herpes simplex virus type 1 in an intensive care nursery. Pediatr. Infect. Dis. 2290-294. - PubMed
    1. Kaneko, H., T. Iida, K. Aoki, S. Ohno, and T. Suzutani. 2005. Sensitive and rapid detection of herpes simplex virus and varicella-zoster virus DNA by loop-mediated isothermal amplification. J. Clin. Microbiol. 433290-3296. - PMC - PubMed

Associated data

LinkOut - more resources