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. 2010 May;48(5):1983-4.
doi: 10.1128/JCM.01862-09. Epub 2010 Mar 10.

Rapid genotypic assay for detection of oseltamivir-resistant influenza A (H1N1) viruses

Mami Hata, Seidai Tanaka, Yoshihiro Yasui, Noriko Fujiwara, Shinichi Kobayashi, Hiroko Minagawa

Rapid genotypic assay for detection of oseltamivir-resistant influenza A (H1N1) viruses

Mami Hata et al. J Clin Microbiol. 2010 May.
No abstract available

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Figures

FIG. 1.
FIG. 1.
MAMA-PCR analysis for detecting a single mutation in the NA genes of oseltamivir-resistant and oseltamivir-sensitive influenza A (H1N1) viruses. Lanes: M, molecular size marker (100-bp ladder); 1, Aichi/92/2007 virus (harboring 275H); 2, Aichi/96/2007 virus (harboring 275H); 3, Aichi/100/2007 virus (harboring 275H); 4, Aichi/74/2008 virus (harboring 275H); 5, Aichi/76/2008 virus (harboring 275Y); 6, Aichi/138/2008 virus (harboring 275Y); 7, Aichi/8/2009 virus (harboring 275Y); and 8, Aichi/29/2009 virus (harboring 275Y). For the reaction mixtures in lanes 1H to 8H, the MAMA275H primer was used, and for those in lanes 1Y to 8Y, the MAMA275Y primer was used. The upper arrow indicates the 938-bp control fragment; the nucleic acid sequence of this fragment was determined. The lower arrow indicates the 539-bp MAMA-PCR product.
See this image and copyright information in PMC

References

    1. Bolotin, S., A. V. Robertson, A. Eshaghi, C. De Lima, E. Lombos, E. Chong-King, L. Burton, T. Mazzulli, and S. J. Drews. 2009. Development of a novel real-time reverse-transcriptase PCR method for the detection of H275Y positive influenza A H1N1 isolates. J. Virol. Methods 158:190-194. - PMC - PubMed
    1. Carr, M. J., N. Sayre, M. Duffy, J. Connell, and W. W. Hall. 2008. Rapid molecular detection of the H275Y oseltamivir resistance gene mutation in circulating influenza A (H1N1) viruses. J. Virol. Methods 153:257-262. - PMC - PubMed
    1. Centers for Disease Control and Prevention. 2009. Outbreak of swine-origin influenza A (H1N1) virus infection—Mexico, March-April 2009. MMWR Morb. Mortal. Wkly. Rep. 58:467-470. - PubMed
    1. Centers for Disease Control and Prevention. 2010. Update: influenza activity—United States, August 30, 2009-January 9, 2010. MMWR Morb. Mortal. Wkly. Rep. 59:38-43. - PubMed
    1. Chen, H., C. L. Cheung, H. Tai, P. Zhao, J. F. Chan, V. C. Cheng, K. H. Chan, and K. Y. Yuen. 2009. Oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus, Hong Kong, China. Emerg. Infect. Dis. 15:1970-1972. - PMC - PubMed

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