Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2013 Oct;51(10):3448-53.
doi: 10.1128/JCM.00737-13. Epub 2013 Jul 24.

Nonsuppurative encephalomyelitis in a calf in Japan and isolation of Japanese encephalitis virus genotype 1 from the affected calf

Takashi Katayama  1 , Sachie Saito, Sanae Horiuchi, Tetsuya Maruta, Tomoko Kato, Tohru Yanase, Makoto Yamakawa, Hiroaki Shirafuji
Affiliations
Case Reports

Nonsuppurative encephalomyelitis in a calf in Japan and isolation of Japanese encephalitis virus genotype 1 from the affected calf

Takashi Katayama et al. J Clin Microbiol. 2013 Oct.

Abstract

Japanese encephalitis virus (JEV) was isolated from the cerebrum of a calf which showed severe neurological symptoms in late September 2009, and the JEV isolate was revealed to be of genotype 1. This is the first report describing the isolation of genotype 1 JEV from cattle.

PubMed Disclaimer

Figures

Fig 1
Fig 1
Phylogenetic profile showing the relationships among JEV isolates based on a comparison of their E regions (1,500 nucleotides). The bootstrap percentages calculated from 1,000 replications are indicated around the internal nodes. The scale represents 0.05% sequence divergence. Mo, mosquito; Hu, human.
Fig 2
Fig 2
Phylogenetic profile showing the relationships among JEV isolates based on a comparison of the complete genome (approximately 11,000 nucleotides). The bootstrap percentages calculated from 1,000 replications are indicated around the internal nodes. The scale represents 0.05% sequence divergence.
Fig 3
Fig 3
Perivascular infiltration of lymphocytes (A) and a glial nodule (B) in the cerebrum of the affected calf. Arrows indicate the perivascular infiltration of lymphocytes, and an arrowhead points out the glial nodule. Hematoxylin and eosin stain. Bar = 200 μm.
Fig 4
Fig 4
IHC assay results. Detection of JEV antigen in the cerebrum (A to C), cerebellum (D), pons (E), and spinal cord (F) of the affected calf. JEV-positive granules (dark brown) are observed mainly in the cytoplasm of neurons and occasionally in nerve axons. (C) Positively labeled neurons are undergoing neuronophagia. Bar = 100 μm.
See this image and copyright information in PMC

References

    1. OIE 2008. Japanese encephalitis, p 231–239 In OIE Biological Standards Commission (ed), Manual of diagnostic tests and vaccines for terrestrial animals, 6th ed, vol 1 Office International des Epizooties, Paris, France
    1. National Institute of Infectious Diseases (NIID) and Japan Association of Prefectural and Municipal Public Health Institutes 2003. Manuals for the detection of pathogens. NIID, Tokyo, Japan: http://www0.nih.go.jp/niid/reference/pathogen-manual-60.pdf Accessed 12 July 2013 (In Japanese.)
    1. Nerome R, Tajima S, Takasaki T, Yoshida T, Kotaki A, Lim CK, Ito M, Sugiyama A, Yamauchi A, Yano T, Kameyama T, Morishita I, Kuwayama M, Ogawa T, Sahara K, Ikegaya A, Kanda M, Hosoya Y, Itokazu K, Onishi H, Chiya S, Yoshida Y, Tabei Y, Katsuki K, Tabata K, Harada S, Kurane I. 2007. Molecular epidemiological analyses of Japanese encephalitis virus isolates from swine in Japan from 2002 to 2004. J. Gen. Virol. 88:2762–2768 - PubMed
    1. Tajima S, Nukui Y, Takasaki T, Kurane I. 2007. Characterization of the variable region in the 3′ non-translated region of dengue type 1 virus. J. Gen. Virol. 88:2214–2222 - PubMed
    1. Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673–4680 - PMC - PubMed

Publication types

Associated data

LinkOut - more resources