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. 1999 Sep;65(9):3976-81.
doi: 10.1128/AEM.65.9.3976-3981.1999.

Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments

K Sahm  1 , C Knoblauch, R Amann
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Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments

K Sahm et al. Appl Environ Microbiol. 1999 Sep.

Abstract

Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the delta subclass of Proteobacteria and were widely distributed within this group, indicating that psychrophily is a polyphyletic property. A new 16S rRNA-directed oligonucleotide probe was designed against the largest coherent cluster of these isolates. The new probe, as well as a set of available probes, was applied in rRNA slot blot hybridization to investigate the composition of the sulfate-reducing bacterial community in the sediments. rRNA related to the new cluster of incompletely oxidizing, psychrophilic isolates made up 1.4 to 20.9% of eubacterial rRNA at Storfjord and 0.6 to 3. 5% of eubacterial rRNA at Hornsund. This group was the second-most-abundant group of sulfate reducers at these sites. Denaturing gradient gel electrophoresis and hybridization analysis showed bands identical to those produced by our isolates. The data indicate that the psychrophilic isolates are quantitatively important in Svalbard sediments.

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Figures

FIG. 1
FIG. 1
Phylogenetic affiliations of psychrophilic sulfate-reducing isolates and specificities of probes used in this study. The tree shows members of the δ subclass of Proteobacteria and was constructed by using the neighbor-joining algorithm and a 50% conservation filter. The arcs comprise the respective probe target groups. Sval428 is described in this study. Other probes are as described by Devereux et al. (7). Strains isolated from Svalbard sediments are shown in boldface type. Strain names indicate the carbon sources on which the strains were isolated (A, acetate; L, lactate; P, propionate; and B, betaine) and the sampling site (Sv2 indicates Hornsund and Sv5 indicates Storfjord).
FIG. 2
FIG. 2
DGGE-hybridization analysis of community 16S rDNA patterns with probe Sval428 for Hornsund sediments. (A) DGGE results; (B) corresponding hybridization results. Numbers give the depth ranges from which target DNAs were extracted. For each depth, two cores have been investigated. “LSv” followed by a number indicates a DGGE fragment of a psychrophilic sulfate-reducing isolate targeted by probe Sval428. The arrow indicates corresponding bands between the community profile and isolates.
See this image and copyright information in PMC

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