Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Jun;66(6):2385-91.
doi: 10.1128/AEM.66.6.2385-2391.2000.

Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene

I M Sulaiman  1 , U M Morgan, R C Thompson, A A Lal, L Xiao
Affiliations

Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene

I M Sulaiman et al. Appl Environ Microbiol. 2000 Jun.

Abstract

We have characterized the nucleotide sequences of the 70-kDa heat shock protein (HSP70) genes of Cryptosporidium baileyi, C. felis, C. meleagridis, C. muris, C. serpentis, C. wrairi, and C. parvum from various animals. Results of the phylogenetic analysis revealed the presence of several genetically distinct species in the genus Cryptosporidium and eight distinct genotypes within the species C. parvum. Some of the latter may represent cryptic species. The phylogenetic tree constructed from these sequences is in agreement with our previous results based on the small-subunit rRNA genes of Cryptosporidium parasites. The Cryptosporidium species formed two major clades: isolates of C. muris and C. serpentis formed the first major group, while isolates of C. felis, C. meleagridis, C. wrairi, and eight genotypes of C. parvum formed the second major group. Sequence variations were also observed between C. muris isolates from ruminants and rodents. The HSP70 gene provides another useful locus for phylogenetic analysis of the genus Cryptosporidium.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Variation in the HSP70 gene nucleotide sequences in the primer regions of diagnostic tools by Rochelle et al. (29) (A and B), Di Giovanni et al. (7) (A and B), and Stinear et al. (33) (C).
FIG. 2
FIG. 2
Phylogenetic relationship of Cryptosporidium parasites to other apicomplexan parasites inferred from neighbor-joining analysis of HSP70 gene nucleotide sequences.
FIG. 3
FIG. 3
Phylogenetic relationships among Cryptosporidium parasites inferred from neighbor-joining analysis of nucleotide sequences (A) and deduced amino acid sequences (B).
See this image and copyright information in PMC

References

    1. Arrowood M J, Sterling C R. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J Parasitol. 1987;73:314–319. - PubMed
    1. Awad-El-Kariem F A, Warhurst D C, McDonald V. Detection of Cryptosporidium oocysts using a system based on PCR and endonuclease restriction. Parasitology. 1994;109:19–22. - PubMed
    1. Budin K, Philippe H. New insights into the phylogeny of eukaryotes based on ciliate Hsp70 sequences. Mol Biol Evol. 1998;15:943–956. - PubMed
    1. Bui E T, Bradley P J, Johnson P J. A common evolutionary origin of the mitochondria and hydrogenosomes. Proc Natl Acad Sci USA. 1996;93:9651–9656. - PMC - PubMed
    1. Champliaud D, Gobet P, Naciri M, Vagner O, Lopez J, Buisson J C, Varga I, Harly G, Mancassola R, Bonnin A. Failure to differentiate Cryptosporidium parvum from C. meleagridis based on PCR amplification of eight DNA sequences. Appl Environ Microbiol. 1998;64:1454–1458. - PMC - PubMed

Publication types

Substances

Associated data

LinkOut - more resources