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. 2002 Oct;184(19):5524-8.
doi: 10.1128/JB.184.19.5524-5528.2002.

A novel gene that bears a DnaJ motif influences cyanobacterial cell division

Olga A Koksharova  1 , C Peter Wolk
Affiliations

A novel gene that bears a DnaJ motif influences cyanobacterial cell division

Olga A Koksharova et al. J Bacteriol. 2002 Oct.

Abstract

Transposon Tn5-692 mutagenizes Synechococcus sp. strain PCC 7942 efficiently. The predicted product of the gene mutated in the Tn5-692-derived cell division mutant FTN2 has an N-terminal DnaJ domain, as have its cyanobacterial and plant orthologs. Anabaena sp. strain PCC 7120, when mutated in genes orthologous to ftn2 and ftn6, forms akinete-like cells.

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Figures

FIG. 1.
FIG. 1.
Morphology of wild-type PCC 7942 (A) and of mutants FTN2 (C) and FTN6 (B), grown in liquid medium and visualized by bright-field light microscopy. Scale bars represent 12.5 μm (A and B) or 25.6 μm (C).
FIG. 2.
FIG. 2.
Structures of wild-type PCC 7942 (A) and of mutants FTN2 (C; see box in panel B) and FTN6 (E; see box in panel D), negatively stained with uranyl acetate and examined by electron microscopy. The cells of both mutants divide infrequently. Scale bars represent 1 μm (A, C, and E) or 10 μm (B and D).
FIG. 3.
FIG. 3.
Morphology of Anabaena sp. strain PCC 7120 wild type (A and B) and mutants FTN2A (C and D) and FTN6A (E and F) grown in liquid medium free of combined nitrogen (AA/8 [20]) (A, C, and E) or supplemented with 5 mM nitrate (B, D, and F). Scale bars represent 12.5 μm. Akinete-like cells are indicated by arrows.
See this image and copyright information in PMC

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