Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2003 May;41(5):2191-6.
doi: 10.1128/JCM.41.5.2191-2196.2003.

Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness

Mamuka Kotetishvili  1 , O Colin Stine, Yuansha Chen, Arnold Kreger, Alexander Sulakvelidze, Shanmuga Sozhamannan, J Glenn Morris Jr
Affiliations
Comparative Study

Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness

Mamuka Kotetishvili et al. J Clin Microbiol. 2003 May.

Abstract

Twenty-two Vibrio cholerae isolates, including some from "epidemic" (O1 and O139) and "nonepidemic" serogroups, were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) by using three housekeeping genes, gyrB, pgm, and recA; sequence data were also obtained for the virulence-associated genes tcpA, ctxA, and ctxB. Even with the small number of loci used, MLST had better discriminatory ability than did PFGE. On MLST analysis, there was clear clustering of epidemic serogroups; much greater diversity was seen among tcpA- and ctxAB-positive V. cholerae strains from other, nonepidemic serogroups, with a number of tcpA and ctxAB alleles identified.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Dendrogram portraying the genetic diversity of various V. cholerae isolates. The dendrogram is based on PFGE patterns obtained with CeuI-digested V. cholerae DNA.
FIG. 2.
FIG. 2.
Dendrogram portraying the genetic diversity among various V. cholerae sequence types, constructed by the unweighted pair group method using averages. Allele types are for tcpA and ctx, respectively.
See this image and copyright information in PMC

References

    1. Beltran, P., G. Delgado, A. Navarro, F. Trujillo, R. K. Selander, and A. Cravioto. 1999. Genetic diversity and population structure of Vibrio cholerae. J. Clin. Microbiol. 37:581-590. - PMC - PubMed
    1. Berche, P., C. Poyart, E. Abachin, H. Lelievre, J. Vandepitte, A. Dodin, and J. M. Fournier. 1994. The novel epidemic isolate O139 is closely related to the pandemic isolate O1 of Vibrio cholerae. J. Infect. Dis. 170:701-704. - PubMed
    1. Bik, E. M., R. D. Gouw, and F. R. Mooi. 1996. DNA fingerprinting of Vibrio cholerae isolates with a novel insertion sequence element: a tool to identify epidemic isolates. J. Clin. Microbiol. 34:1453-1461. - PMC - PubMed
    1. Boyd, E. F., and M. K. Waldor. 2002. Evolutionary and functional analyses of variants of the toxin-coregulated pilus protein TcpA from toxigenic Vibrio cholerae non-O1/non-O139 serogroup isolates. Microbiology 148:1655-1666. - PubMed
    1. Byun, R., L. D. H. Elbourne, R. Lan, and P. R. Reeves. 1999. Evolutionary relationships of pathogenic clones of Vibrio cholerae by sequence analysis of four housekeeping genes. Infect. Immun. 67:1116-1124. - PMC - PubMed

Publication types

Associated data

LinkOut - more resources