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. 2020 Mar;26(2):341-349.
doi: 10.1111/odi.13230. Epub 2019 Dec 17.

Immunohistochemistry and gene expression of GLUT1, RUNX2 and MTOR in reparative dentinogenesis

Ryosuke Takeuchi  1 , Naoto Ohkura  1 , Kunihiko Yoshiba  2 , Aiko Tohma  1 , Nagako Yoshiba  1 , Naoki Edanami  1 , Mari Shirakashi  1 , Razi Saifullah Ibn Belal  1 , Hayato Ohshima  3 , Yuichiro Noiri  1
Affiliations

Immunohistochemistry and gene expression of GLUT1, RUNX2 and MTOR in reparative dentinogenesis

Ryosuke Takeuchi et al. Oral Dis. 2020 Mar.

Abstract

Objectives: To determine glucose transporter 1 (GLUT1) and runt-related transcription factor 2 (RUNX2) expression during reparative dentinogenesis after pulpotomy with mineral trioxide aggregate (MTA) capping.

Subjects and methods: Eight-week-old male Wistar rats were used. Pulp of the upper left first molar was exposed and capped with MTA. The upper right first molar of the same animal was used as a control. After collecting molars at various time points, GLUT1, RUNX2 and mammalian target of rapamycin (MTOR) were examined by immunohistochemistry. mRNA levels of Slc2a1 (encoding GLUT1), Runx2, Nestin and Mtor were determined by real-time PCR.

Results: Pulp exhibited progressive formation of reparative dentine lined with GLUT1- and MTOR-immunoreactive odontoblast-like cells at 5 days after pulpotomy. RUNX2 was detected in nuclei of most pulp tissue cells at day 5 after pulpotomy. Double immunofluorescence staining revealed GLUT1 immunoreactivity on odontoblast-like cells positive for Nestin or RUNX2, 5 days after pulpotomy. Slc2a1, Runx2, Nestin and Mtor mRNA levels were significantly upregulated on days 3-5 after pulpotomy.

Conclusions: After rat molar pulpotomy, dental pulp induced formation of reparative dentine with colocalization of GLUT1 and Nestin or RUNX2. Moreover, mRNA levels of Slc2a1, Runx2, Nestin and Mtor were significantly upregulated in pulpotomized dental pulp.

Keywords: glucose transporter 1; mammalian target of rapamycin; mineral trioxide aggregate; odontoblast-like cell; reparative dentine; runt-related transcription factor 2.

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References

REFERENCES

    1. About, I., Laurent-Maquin, D., Lendahl, U., & Mitsiadis, T. A. (2000). Nestin expression in embryonic and adult human teeth under normal and pathological conditions. American Journal of Pathology, 157(1), 287-295. https://doi.org/10.1016/S0002-9440(10)64539-7
    1. Baume, L. J., & Holz, J. (1981). Long term clinical assessment of direct pulp capping. International Dental Journal, 31(4), 251-260.
    1. Bronckers, A. L., Engelse, M. A., Cavender, A., Gaikwad, J., & D'Souza, R. N. (2001). Cell-specific patterns of Cbfa1 mRNA and protein expression in postnatal murine dental tissues. Mechanisms of Development, 101(1-2), 255-258. https://doi.org/10.1016/S0925-4773(00)00562-1
    1. Chen, S., Gluhak-Heinrich, J., Wang, Y. H., Wu, Y. M., Chuang, H. H., Chen, L., … MacDougall, M. (2009). Runx2, osx, and dspp in tooth development. Journal of Dental Research, 88(10), 904-909. https://doi.org/10.1177/0022034509342873
    1. Chu, Q., Gao, Y., Gao, X., Dong, Z., Song, W., Xu, Z., … Gao, Y. (2018). Ablation of Runx2 in ameloblasts suppresses enamel maturation in tooth development. Scientific Reports, 8(1), 9594. https://doi.org/10.1038/s41598-018-27873-5

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