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. 2002 Mar 8;108(5):637-48.
doi: 10.1016/s0092-8674(02)00647-5.

Trypanosome mitochondrial 3' terminal uridylyl transferase (TUTase): the key enzyme in U-insertion/deletion RNA editing

Ruslan Aphasizhev  1 , Sandro Sbicego, Marian Peris, Sei-Heon Jang, Inna Aphasizheva, Agda M Simpson, Anatoly Rivlin, Larry Simpson
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Free article

Trypanosome mitochondrial 3' terminal uridylyl transferase (TUTase): the key enzyme in U-insertion/deletion RNA editing

Ruslan Aphasizhev et al. Cell. .
Free article

Erratum in

  • Cell 2002 Jul 12;110(1):133

Abstract

A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg(2+). TUTase is present in vivo in at least two stable configurations: one contains a approximately 500 kDa TUTase oligomer and the other a approximately 700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.

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