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Comparative Study
. 2003 Dec;69(12):7517-22.
doi: 10.1128/AEM.69.12.7517-7522.2003.

Comparative sequence analysis of the tuf and recA genes and restriction fragment length polymorphism of the internal transcribed spacer region sequences supply additional tools for discriminating Bifidobacterium lactis from Bifidobacterium animalis

Marco Ventura  1 , Ralf Zink
Affiliations
Comparative Study

Comparative sequence analysis of the tuf and recA genes and restriction fragment length polymorphism of the internal transcribed spacer region sequences supply additional tools for discriminating Bifidobacterium lactis from Bifidobacterium animalis

Marco Ventura et al. Appl Environ Microbiol. 2003 Dec.

Abstract

The relationship between Bifidobacterium lactis and Bifidobacterium animalis was examined by comparative analysis of tuf and recA gene sequences and by restriction fragment length polymorphism analysis of their internal 16S-23S transcribed spacer region sequences. The bifidobacterial strains investigated could be divided into two distinct groups within a single species based on the tuf, recA, and 16S-23S spacer region sequence analysis. Therefore, all strains of B. lactis and B. animalis could be unified as the species B. animalis and divided into two subspecies, Bifidobacterium animalis subsp. lactis and Bifidobacterium animalis subsp. animalis.

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Figures

FIG. 1.
FIG. 1.
(a and b) Phylogenetic trees obtained with tuf gene sequences (a) and recA gene sequences (b). The scale indicates a genetic distances of 2%. (c and d) Alignment showing the sequence signatures for the tuf gene sequences (c) and recA gene sequences (d). Sequence signatures are shaded in black; residues shaded in light grey have ≤75% identity, while residues in dark grey have ≥76% identity.
FIG. 2.
FIG. 2.
(a and b) Pairwise alignment of the tuf sequences (a) and recA sequences (b) of B. lactis DSM 10140T and B. animalis ATCC 25527T. Nucleotides which are different between B. lactis strains and B. animalis ATCC 25527 are shaded in black. (c and d) Theoretical RFLP profiles from B. lactis-B. animalis tuf sequences (c) and from B. lactis-B. animalis recA sequences (d).
FIG. 3.
FIG. 3.
Restriction patterns of PCR-amplified fragments of 16S-23S rDNA digested with Sau3AI. Lane 1, B. animalis ATCC 25527; lane 2, B. animalis ATCC 27672; lane 3, B. lactis DSM 10140; lane 4, B. animalis ATCC 27674; lane 5, B. animalis ATCC 27673; lane 6, B. animalis ATCC 27536; lane 7, B. lactis NCC 363; lane 8, B. lactis NCC 311; lane 9, B. lactis NCC 402; lane 10, B. lactis NCC 311; lane 11, B. lactis NCC 383; lane 12, B. lactis NCC 239; lanes M, 50-bp DNA ladder (Gibco BRL).
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References

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