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. 2004 Jun;42(6):2802-5.
doi: 10.1128/JCM.42.6.2802-2805.2004.

Molecular characterization and phylogenetic study of newcastle disease virus isolates from recent outbreaks in eastern Uganda

Maxwell O Otim  1 , Henrik Christensen, Poul H Jørgensen, Kurt J Handberg, Magne Bisgaard
Affiliations

Molecular characterization and phylogenetic study of newcastle disease virus isolates from recent outbreaks in eastern Uganda

Maxwell O Otim et al. J Clin Microbiol. 2004 Jun.

Abstract

Newcastle disease virus isolates from chickens in eastern Uganda in 2001 were found to be velogenic by fusion protein cleavage site sequence analysis and biological characterization; the intracerebral pathogenicity index was 1.8. Analysis of their hemagglutinin-neuraminidase protein gene sequences revealed a novel genotype unrelated to those that caused previous outbreaks.

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Figures

FIG. 1.
FIG. 1.
F protein cleavage site. All 16 Ugandan NDV isolates had the same amino acid residues (RRQKRFVG) around the cleavage site, which is indicated by an arrow. The sequence in bold represents the F protein cleavage site motif.
FIG. 2.
FIG. 2.
Phylogenetic tree of the nucleotide sequences of the Ugandan NDV isolates based on a 377-bp region of the HN protein gene. Genotypes and subtypes are indicated at the right. Numbers at nodes indicate support by bootstrap resampling of 100 replicates.
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