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. 2005 Dec;43(12):6176-82.
doi: 10.1128/JCM.43.12.6176-6182.2005.

Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region

I Casas  1 , A Avellon, M Mosquera, O Jabado, J E Echevarria, R H Campos, M Rewers, P Perez-Breña, W I Lipkin, G Palacios
Affiliations

Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region

I Casas et al. J Clin Microbiol. 2005 Dec.

Abstract

Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.

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Figures

FIG. 1.
FIG. 1.
Nucleotide sequences were aligned by using Clustal W. Phylogenetic analyses were performed using the Kimura two-parameter model as a model of nucleotide substitution and using the neighbor-joining method to reconstruct phylogenetic trees (MEGA version 2.1 software package). The statistical significance of the phylogenies constructed was estimated by bootstrap analysis with 1,000 pseudoreplicate data sets.
FIG. 1.
FIG. 1.
Nucleotide sequences were aligned by using Clustal W. Phylogenetic analyses were performed using the Kimura two-parameter model as a model of nucleotide substitution and using the neighbor-joining method to reconstruct phylogenetic trees (MEGA version 2.1 software package). The statistical significance of the phylogenies constructed was estimated by bootstrap analysis with 1,000 pseudoreplicate data sets.
FIG. 1.
FIG. 1.
Nucleotide sequences were aligned by using Clustal W. Phylogenetic analyses were performed using the Kimura two-parameter model as a model of nucleotide substitution and using the neighbor-joining method to reconstruct phylogenetic trees (MEGA version 2.1 software package). The statistical significance of the phylogenies constructed was estimated by bootstrap analysis with 1,000 pseudoreplicate data sets.
See this image and copyright information in PMC

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