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1959 24
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1963 29
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1966 11
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1968 12
1969 15
1970 15
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1974 5
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1977 6
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1980 8
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1982 21
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1986 14
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1988 20
1989 18
1990 12
1991 15
1992 22
1993 16
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1996 27
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1998 17
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2000 18
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2002 43
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2005 40
2006 59
2007 62
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2009 74
2010 79
2011 62
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2,900 results

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Page 1
Developments in FRET- and BRET-Based Biosensors.
Wu Y, Jiang T. Wu Y, et al. Micromachines (Basel). 2022 Oct 20;13(10):1789. doi: 10.3390/mi13101789. Micromachines (Basel). 2022. PMID: 36296141 Free PMC article. Review.
Here, we provide an overview of recent progress on FRET- and BRET-based biosensors and their roles in biomedicine, environmental applications, and synthetic biology. This review highlights FRET- and BRET-based biosensors and gives examples of their applications with …
Here, we provide an overview of recent progress on FRET- and BRET-based biosensors and their roles in biomedicine, environmental appl …
The BRET technology and its application to screening assays.
Bacart J, Corbel C, Jockers R, Bach S, Couturier C. Bacart J, et al. Biotechnol J. 2008 Mar;3(3):311-24. doi: 10.1002/biot.200700222. Biotechnol J. 2008. PMID: 18228541 Review.
Various studies have applied BRET-based assays to screen new receptor ligands and inhibitors of disease-related-proteases. ...This review describes the original BRET-based methodology, more recent variants, and potential applications to drug screening....
Various studies have applied BRET-based assays to screen new receptor ligands and inhibitors of disease-related-proteases. ...This re …
Conformation- and activation-based BRET sensors differentially report on GPCR-G protein coupling.
Wright SC, Avet C, Gaitonde SA, Muneta-Arrate I, Le Gouill C, Hogue M, Breton B, Koutsilieri S, Diez-Alarcia R, Héroux M, Lauschke VM, Bouvier M. Wright SC, et al. Sci Signal. 2024 Jun 18;17(841):eadi4747. doi: 10.1126/scisignal.adi4747. Epub 2024 Jun 18. Sci Signal. 2024. PMID: 38889226
Biosensors based on bioluminescence resonance energy transfer (BRET) have advanced our understanding of GPCR functional selectivity. Some BRET biosensors monitor ligand-induced conformational changes in the receptor or G proteins, whereas others monitor the recruitm …
Biosensors based on bioluminescence resonance energy transfer (BRET) have advanced our understanding of GPCR functional selectivity. …
Application of BRET for studying G protein-coupled receptors.
Kaczor AA, Makarska-Bialokoz M, Selent J, de la Fuente RA, Martí-Solano M, Castro M. Kaczor AA, et al. Mini Rev Med Chem. 2014 May;14(5):411-25. doi: 10.2174/1389557514666140428113708. Mini Rev Med Chem. 2014. PMID: 24766382 Review.
Data from numerous BRET experiments support the idea that the process of GPCR oligomerization may be relevant in many physiological and pathological conditions. ...In this review, we briefly summarize the fundaments of BRET, discuss new trends in this technology and …
Data from numerous BRET experiments support the idea that the process of GPCR oligomerization may be relevant in many physiological a …
BRET-Based Self-Cleaving Biosensors for SARS-CoV-2 3CLpro Inhibitor Discovery.
Hou N, Peng C, Zhang L, Zhu Y, Hu Q. Hou N, et al. Microbiol Spectr. 2022 Aug 31;10(4):e0255921. doi: 10.1128/spectrum.02559-21. Epub 2022 Jun 27. Microbiol Spectr. 2022. PMID: 35758897 Free PMC article.
These inhibitors restored the BRET signal by blocking the proteolysis of pBRET-10, and their relative activities measured using pBRET-10 were consistent with their previously reported anti-SARS-CoV-2 activities. ...However, the development of 3CLpro inhibitors relies heavi …
These inhibitors restored the BRET signal by blocking the proteolysis of pBRET-10, and their relative activities measured using pBRET …
Monitoring Ligand-Activated Protein-Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay.
Coriano C, Powell E, Xu W. Coriano C, et al. Methods Mol Biol. 2016;1473:3-15. doi: 10.1007/978-1-4939-6346-1_1. Methods Mol Biol. 2016. PMID: 27518618 Review.
This chapter focuses on describing the toxicological applications of BRET and its optimization as a high-throughput detection system in live cells. Here we review the construction of BRET fusion proteins, describe the BRET methodology, and outline strategies …
This chapter focuses on describing the toxicological applications of BRET and its optimization as a high-throughput detection system …
Application of FRET- and BRET-based live-cell biosensors in deorphanization and ligand discovery studies on orphan G protein-coupled receptors.
Sajkowska JJ, Tsang CH, Kozielewicz P. Sajkowska JJ, et al. SLAS Discov. 2024 Sep;29(6):100174. doi: 10.1016/j.slasd.2024.100174. Epub 2024 Jul 29. SLAS Discov. 2024. PMID: 39084335 Free article. Review.
In this review, the cases where biophysical methods, BRET and FRET, were employed for deorphanization and ligand discovery studies on orphan G protein-coupled receptors are listed and discussed....
In this review, the cases where biophysical methods, BRET and FRET, were employed for deorphanization and ligand discovery studies on …
BRET measurement on CCD camera-based microtiter plate readers.
Cho KF, Javier N, Choi K. Cho KF, et al. SLAS Discov. 2022 Oct;27(7):413-417. doi: 10.1016/j.slasd.2022.08.002. Epub 2022 Aug 15. SLAS Discov. 2022. PMID: 35981684 Free article.
NanoBRET assays, which utilize bioluminescence energy resonance transfer (BRET), have been widely adopted in drug discovery for measuring both protein-protein interactions and drug target engagement. ...
NanoBRET assays, which utilize bioluminescence energy resonance transfer (BRET), have been widely adopted in drug discovery for measu …
Engineering BRET-Sensor Proteins.
Arts R, Aper SJ, Merkx M. Arts R, et al. Methods Enzymol. 2017;589:87-114. doi: 10.1016/bs.mie.2017.01.010. Epub 2017 Feb 20. Methods Enzymol. 2017. PMID: 28336075
As a result, the application of BRET-sensors is no longer restricted to measurements on cell populations, but they can also be used for imaging of single living cells, and BRET has started to emerge as an attractive sensor format for point-of-care diagnostics. The a …
As a result, the application of BRET-sensors is no longer restricted to measurements on cell populations, but they can also be used f …
BRET-Based Dual-Color (Visible/Near-Infrared) Molecular Imaging Using a Quantum Dot/EGFP-Luciferase Conjugate.
Tsuboi S, Jin T. Tsuboi S, et al. Methods Mol Biol. 2022;2525:47-59. doi: 10.1007/978-1-0716-2473-9_5. Methods Mol Biol. 2022. PMID: 35836060
Here we present bioluminescence resonance energy transfer (BRET)-based visible and near-infrared dual-color molecular imaging using a quantum dot (QD) and luciferase-protein conjugate....
Here we present bioluminescence resonance energy transfer (BRET)-based visible and near-infrared dual-color molecular imaging using a …
2,900 results