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Comparative Study
. 2003;102(1-4):347-54.
doi: 10.1159/000075773.

Sequence analysis of a rainbow trout cDNA library and creation of a gene index

C E Rexroad 3rd  1 , Y Lee, J W Keele, S Karamycheva, G Brown, B Koop, S A Gahr, Y Palti, J Quackenbush
Affiliations
Comparative Study

Sequence analysis of a rainbow trout cDNA library and creation of a gene index

C E Rexroad 3rd et al. Cytogenet Genome Res. 2003.

Abstract

Expressed sequence tag (EST) projects have produced extremely valuable resources for identifying genes affecting phenotypes of interest. A large-scale EST sequencing project for rainbow trout was initiated to identify and functionally annotate as many unique transcripts as possible. Over 45,000 5' ESTs were obtained by sequencing clones from a single normalized library constructed using mRNA from six tissues. The production of this sequence data and creation of a rainbow trout Gene Index eliminating redundancy and providing annotation for these sequences will facilitate research in this species.

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