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Comparative Study
. 2015 Mar;362(5):fnv011.
doi: 10.1093/femsle/fnv011. Epub 2015 Jan 28.

The genome of Shigella dysenteriae strain Sd1617 comparison to representative strains in evaluating pathogenesis

Ajchara A Vongsawan  1 , Vinayak Kapatral  2 , Benjamin Vaisvil  2 , Henry Burd  2 , Oralak Serichantalergs  3 , Malabi M Venkatesan  4 , Carl J Mason  3
Affiliations
Comparative Study

The genome of Shigella dysenteriae strain Sd1617 comparison to representative strains in evaluating pathogenesis

Ajchara A Vongsawan et al. FEMS Microbiol Lett. 2015 Mar.

Abstract

We sequenced and analyzed Shigella dysenteriae strain Sd1617 serotype 1 that is widely used as model strain for vaccine design, trials and research. A combination of next-generation sequencing platforms and assembly yielded two contigs representing a chromosome size of 4.34 Mb and the large virulence plasmid of 177 kb. This genome sequence is compared with other Shigella genomes in order to understand gene complexity and pathogenic factors.

Keywords: Shigella dysenteriae; comparison; genome.

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Figures

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The Shigella dysenteriae strain Sd1617 serotype 1 has been sequenced and analyzed. It is widely used as model strain for vaccine design, trials and research. A combination of next-generation sequencing platforms and assembly yielded two contigs representing a chromosome size of 4.34 Mb and the large virulence plasmid of 177 kb.
Figure 1.
Figure 1.
The ORF OmpA is located in an IS element region in all Shigella genomes. Similar colored ORFs have identical functions. (1) The IS elements are shown in dashed boxes. The ORFS are (1) helicase, (2) membrane protein, (3) hypothetical protein, (5–7) transposase, (8) hypothetical protein, (9) OmpA, (10) YcgB protein, (11) Lon protease (frameshift in strain Sd 1917) and (12) FabA protein.
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