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. 2012 Jan;28(1):110-4.
doi: 10.1089/AID.2011.0117. Epub 2011 Jun 24.

Phylogenetic and similarity analysis of HTLV-1 isolates from HIV-coinfected patients from the south and southeast regions of Brazil

Mariana Cavalheiro Magri  1 , Luis Fernando de Macedo Brigido, Rosangela Rodrigues, Helena Kaminami Morimoto, João Leandro de Paula Ferreira, Adele Caterino-de-Araujo
Affiliations

Phylogenetic and similarity analysis of HTLV-1 isolates from HIV-coinfected patients from the south and southeast regions of Brazil

Mariana Cavalheiro Magri et al. AIDS Res Hum Retroviruses. 2012 Jan.

Abstract

HTLV-1 is endemic in Brazil and HIV/HTLV-1 coinfection has been detected, mostly in the northeast region. Cosmopolitan HTLV-1a is the main subtype that circulates in Brazil. This study characterized 17 HTLV-1 isolates from HIV coinfected patients of southern (n=7) and southeastern (n=10) Brazil. HTLV-1 provirus DNA was amplified by nested PCR (env and LTR) and sequenced. Env sequences (705 bp) from 15 isolates and LTR sequences (731 bp) from 17 isolates showed 99.5% and 98.8% similarity among sequences, respectively. Comparing these sequences with ATK (HTLV-1a) and Mel5 (HTLV-1c) prototypes, similarities of 99% and 97.4%, respectively, for env and LTR with ATK, and 91.6% and 90.3% with Mel5, were detected. Phylogenetic analysis showed that all sequences belonged to the transcontinental subgroup A of the Cosmopolitan subtype, clustering in two Latin American clusters.

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Figures

FIG. 1.
FIG. 1.
Phylogenetic tree constructed by the maximum-likelihood method using the PAUP v4b10 software for the partial env region of 705 bp (position 5614–6318 relative to the ATK prototype) of HTLV-1 of 44 isolates, including sequences from the south and southeast regions of Brazil (GenBank AN HM770426-HM770440) in bold, generated with the TrN + G model. Bootstrap values above 70% and zero length using the likelihood ratio test with p<0.001 (**) and p≤0.05 (*) in key branches are depicted. The HTLV-1 Mel5 isolate was used as the outgroup.
FIG. 2.
FIG. 2.
Phylogenetic tree constructed by the maximum-likelihood method using the PAUP v4b10 software for the partial LTR region of 692 bp (position 75–766 relative to the ATK prototype) of HTLV-1 of 59 isolates, including sequences from the south and southeast regions of Brazil (GenBank AN JF271836-JF271852) in bold, generated with the GTR + G model. Bootstrap values above 70% and zero length using the likelihood ratio test with p<0.001 (**) and p≤0.05 (*) in key branches are depicted. The HTLV-1 Mel5 isolate was used as the outgroup.
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