Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Dec;76(24):8269-76.
doi: 10.1128/AEM.00687-10. Epub 2010 Oct 22.

Multiple unique Cryptosporidium isolates from three species of ground squirrels (Spermophilus beecheyi, S. beldingi, and S. lateralis) in California

Maria das Graças Cabral Pereira  1 , Xunde Li, Brenda McCowan, Ralph L Phillips, Edward R Atwill
Affiliations

Multiple unique Cryptosporidium isolates from three species of ground squirrels (Spermophilus beecheyi, S. beldingi, and S. lateralis) in California

Maria das Graças Cabral Pereira et al. Appl Environ Microbiol. 2010 Dec.

Abstract

Phylogenetic analyses of the 18S small-subunit (SSU) rRNA and Cryptosporidium oocyst wall protein (COWP) genes indicate that multiple unique Cryptosporidium isolates are shed by separate species of Spermophilus ground squirrels (S. beecheyi, S. beldingi, and S. lateralis) throughout California, despite some squirrel populations' being geographically isolated. The data support our assertion that Spermophilus squirrels shed novel Cryptosporidium species.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Geographic locations of three different species of Spermophilus ground squirrels from which 49 isolates of Cryptosporidium were collected in California from 2000 to 2004.
FIG. 2.
FIG. 2.
Phylogenetic relationships of partial (∼750-bp) 18S SSU rRNA sequences from Cryptosporidium species, genotypes, and novel Cryptosporidium isolates from three species of Spermophilus ground squirrels, S. beecheyi, S. lateralis, and S. beldingi, inferred by neighbor-joining analyses with 1,000 bootstrap replicates.
FIG. 3.
FIG. 3.
Phylogenetic relationships of the entire 18S SSU rRNA sequences (∼1,750 bp) from Cryptosporidium species, genotypes, and novel Cryptosporidium isolates from three species of Spermophilus ground squirrels, S. beecheyi, S. lateralis, and S. beldingi, inferred by neighbor-joining analyses with 1,000 bootstrap replicates.
See this image and copyright information in PMC

References

    1. Atwill, E. R., R. Phillips, M. G. C. Pereira, X. Li, and B. McCowan. 2004. Seasonal shedding of multiple Cryptosporidium genotypes in California ground squirrels (Spermophilus beecheyi). Appl. Environ. Microbiol. 70:6748-6752. - PMC - PubMed
    1. Atwill, E. R., R. Phillips, and F. Rulofson. 2002. Environmental loading rates of the waterborne pathogenic protozoa Cryptosporidium parvum in certain domestic and wildlife species in California, p. 241-243. In R. M. Timm and R. H. Schmidt (ed.), Proceedings of the 20th Vertebrate Pest Conference, Reno, Nevada.
    1. Atwill, E. R., S. M. Camargo, R. Phillips, L. H. Alonso, K. W. Tate, W. A. Jensen, J. Bennet, S. Little, and T. P. Salmon. 2001. Quantitative shedding of two genotypes of Crypstosporidium parvum in California ground squirrels (Spermophilus beecheyi). Appl. Environ. Microbiol. 67:2840-2843. - PMC - PubMed
    1. Current, W. L. 1989. Cryptosporidium spp., p. 281-341. In P. D. Walzer and R. M. Genta (ed.), Parasitic infections in the compromised host. Marcel Dekker, New York, NY.
    1. da Silva, A. J., S. Cacciò, C. Williams, K. Y. Won, E. K. Nace, C. Whittier, N. J. Pieniazek, and M. L. Eberhard. 2003. Molecular and morphologic characterization of a Cryptosporidium genotype identified in lemurs. Vet. Parasitol. 111:297-307. - PubMed

Publication types

MeSH terms