Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Sep;8(3):299-301.
doi: 10.4142/jvs.2007.8.3.299.

Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus)

Hyun Jeong Jeong  1 , Joong Bok Lee, Seung Yong Park, Chang Seon Song, Bo Sook Kim, Jung Rae Rho, Mi Hyun Yoo, Byung Hoon Jeong, Yong Sun Kim, In Soo Choi
Affiliations

Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus)

Hyun Jeong Jeong et al. J Vet Sci. 2007 Sep.

Abstract

Polymorphisms of the prion protein gene (PRNP) have been detected in several cervid species. In order to confirm the genetic variations, this study examined the DNA sequences of the PRNP obtained from 33 captive sika deer (Cervus nippon laiouanus) in Korea. A total of three single-nucleotide polymorphisms (SNPs) at codons 100, 136 and 226 in the PRNP of the sika deer were identified. The polymorphic site located at codon 100 has not been reported. The SNPs detected at codons 100 and 226 induced amino acid substitutions. The SNP at codon 136 was a silent mutation that does not induce any amino acid change. The genotype and allele frequencies were determined for each of the SNPs.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Amplification of the sika deer prion gene (PRNP). M; 100 bp DNA ladder, lane 1; 771 bp of the sika deer PRNP.
Fig. 2
Fig. 2
Single-nucleotide polymorphism at codon 100 in the sika deer PRNP. Electropherograms show two genotypes at codon 100 in the sika deer PRNP. Left panel; AGT/AGT (Ser/Ser), right panel; AGT/GGT (Ser/Gly). No GGT/GGT (Gly/Gly) homozygote was detected in this study.
Fig. 3
Fig. 3
Single-nucleotide polymorphism at codon 136 in the sika deer PRNP. The electropherograms demonstrate three genotypes at codon 136 in the sika deer PRNP. Left panel; GCT/GCT (Ala/Ala), middle panel; GCT/GCC (Ala/Ala), right panel; GCC/GCC (Ala/Ala).
Fig. 4
Fig. 4
Single-nucleotide polymorphism at codon 226 in the sika deer PRNP. The electropherograms show three genotypes at codon 226 in the sika deer PRNP. Left panel; CAG/CAG (Gln/Gln), middle panel; CAG/GAG (Gln/Glu), right panel; GAG/GAG (Glu/Glu).
See this image and copyright information in PMC

References

    1. Collinge J, Sidle KC, Meads J, Ironside J, Hill AF. Molecular analysis of prion strain variation and the aetiology of 'new variant' CJD. Nature. 1996;383:685–690. - PubMed
    1. Hunter N, Goldmann W, Foster JD, Cairns D, Smith G. Natural scrapie and PrP genotype: case-control studies in British sheep. Vet Rec. 1997;141:137–140. - PubMed
    1. Jewell JE, Conner MM, Wolfe LL, Miller MW, Williams ES. Low frequency of PrP genotype 225SF among free-ranging mule deer (Odocoileus hemionus) with chronic wasting disease. J Gen Virol. 2005;86:2127–2134. - PubMed
    1. Johnson C, Johnson J, Vanderloo JP, Keane D, Aiken JM, McKenzie D. Prion protein polymorphisms in white-tailed deer influence susceptibility to chronic wasting disease. J Gen Virol. 2006;87:2109–2114. - PubMed
    1. Kataoka N, Nishimura M, Horiuchi M, Ishiguro N. Surveillance of chronic wasting disease in sika deer, Cervus nippon, from Tokachi district in Hokkaido. J Vet Med Sci. 2005;67:349–351. - PubMed

Publication types

Associated data