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. 2008 Jan;74(1):329-32.
doi: 10.1128/AEM.01448-07. Epub 2007 Nov 9.

Eucaryotic diversity in a hypersaline microbial mat

Leah M Feazel  1 , John R Spear, Alicia B Berger, J Kirk Harris, Daniel N Frank, Ruth E Ley, Norman R Pace
Affiliations

Eucaryotic diversity in a hypersaline microbial mat

Leah M Feazel et al. Appl Environ Microbiol. 2008 Jan.

Abstract

To determine the eucaryotic diversity of the hypersaline Guerrero Negro microbial mat, we amplified 18S rRNA genes from DNA extracted from this mat and constructed and analyzed clone libraries. The extent of eucaryotic diversity detected was remarkably low, only 15 species among 890 clones analyzed. Six eucaryotic kingdoms were represented, as well as a novel cluster of sequences. Nematode sequences dominated the clone libraries.

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Figures

FIG. 1.
FIG. 1.
Guerrero Negro eucaryotic diversity. This pie chart shows the relative diversity of each of the eucaryotic species detected in the mat, based on the 890 clones screened.
FIG. 2.
FIG. 2.
Nematode phylogeny. This phylogenetic tree shows the phylogenetic relationships between the nematodes sequenced in this study and previously studied nematode species. Scanning electron microscopy images of a nematode from the mat, a fully expanded nematode phylogeny, and more images are available in the supplemental material online.
See this image and copyright information in PMC

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