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. 2008 Feb;46(2):759-62.
doi: 10.1128/JCM.02009-07. Epub 2007 Dec 12.

Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures

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Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures

Michel de Vries et al. J Clin Microbiol. 2008 Feb.

Abstract

Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.

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Figures

FIG. 1.
FIG. 1.
Alignment of predicted amino acid sequence in the C terminus of the VP1 protein. The RGD motif is indicated by a black background. The insertion of 3 amino acids, located 7 amino acids upstream of the RGD motif, is marked with gray.

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