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. 2010 Jan 12:9:1.
doi: 10.1186/1476-0711-9-1.

Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria

Murat Kasap  1 , Kayode Fashae, Sinem Torol, Fetiye Kolayli, Fatma Budak, Haluk Vahaboglu
Affiliations

Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in Nigeria

Murat Kasap et al. Ann Clin Microbiol Antimicrob. .

Abstract

Background: We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.

Methods: Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.

Results: By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26.

Conclusion: This is the first study demonstrated the occurrence of SHV-12 in Nigeria.

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Figures

Figure 1
Figure 1
The schematic map of the cloned insert (5656 bp). ORFs are indicated by empty arrows and repeat regions by dark shaded boxes.
Figure 2
Figure 2
IEF in ampholyte gradient of pH 3 to 10 with crude extracts. Lane 1, enzymes (pIs, 5.4 & ≈ 9.0) from E. aerogenes (EaN146); lane 2, enzyme (pI 8.2) from the clone (CN146) and lane 3, enzymes (pIs, 5.4 & ≈ 8.9) from the transformant (TN146). The enzymes at pI 9 in lane 1 and pI 8.9 in lane 3 represented chromosomal enzymes (AmpC).
See this image and copyright information in PMC

References

    1. Chow JW, Fine MJ, Shlaes DM, Quinn JP, Hooper DC, Johnson MP, Ramphal R, Wagener MM, Miyashiro DK, Yu VL. Enterobacter bacteremia: clinical features and emergence of antibiotic resistance during therapy. Annals of internal medicine. 1991;115:585–590. - PubMed
    1. Pfaller MA, Jones RN, Marshall SA, Coffman SL, Hollis RJ, Edmond MB, Wenzel RP. Inducible amp C beta-lactamase producing gram-negative bacilli from blood stream infections: frequency, antimicrobial susceptibility, and molecular epidemiology in a national surveillance program (SCOPE) Diagn Microbiol Infect Dis. 1997;28:211–219. doi: 10.1016/S0732-8893(97)00064-3. - DOI - PubMed
    1. Tzelepi E, Giakkoupi P, Sofianou D, Loukova V, Kemeroglou A, Tsakris A. Detection of extended-spectrum beta-lactamases in clinical isolates of Enterobacter cloacae and Enterobacter aerogenes. J Clin Microbiol. 2000;38:542–546. - PMC - PubMed
    1. Partridge SR, Hall RM. Evolution of transposons containing blaTEM genes. Antimicrob Agents Chemother. 2005;49:1267–1268. doi: 10.1128/AAC.49.3.1267-1268.2005. - DOI - PMC - PubMed
    1. Chaves J, Ladona MG, Segura C, Coira A, Reig R, Ampurdanes C. SHV-1 beta-lactamase is mainly a chromosomally encoded species-specific enzyme in Klebsiella pneumoniae. Antimicrob Agents Chemother. 2001;45:2856–2861. doi: 10.1128/AAC.45.10.2856-2861.2001. - DOI - PMC - PubMed

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