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357,804 results

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Page 1
Direct ELISA.
Lin AV. Lin AV. Methods Mol Biol. 2015;1318:61-7. doi: 10.1007/978-1-4939-2742-5_6. Methods Mol Biol. 2015. PMID: 26160564
First described by Engvall and Perlmann, the enzyme-linked immunosorbent assay (ELISA) is a rapid and sensitive method for detection and quantitation of an antigen using an enzyme-labeled antibody. ...Depending on the antigen epitope and availab …
First described by Engvall and Perlmann, the enzyme-linked immunosorbent assay (ELISA) is a rapid and sen …
[ELISA for diagnosis of infections by viruses].
Yoshihara N. Yoshihara N. Nihon Rinsho. 1995 Sep;53(9):2277-82. Nihon Rinsho. 1995. PMID: 7474393 Review. Japanese.
HBV, HCV, HIV and HTLV, is the enzyme linked immunosorbent assay (ELISA), whose sensitivity and practicability have rendered it the most common primary screening assay. ELISA can be mass screening used automatic or semiautomatic ma …
HBV, HCV, HIV and HTLV, is the enzyme linked immunosorbent assay (ELISA), whose sensitivity and practicab …
ELISA versus PCR for diagnosis of chronic Chagas disease: systematic review and meta-analysis.
Brasil PE, De Castro L, Hasslocher-Moreno AM, Sangenis LH, Braga JU. Brasil PE, et al. BMC Infect Dis. 2010 Nov 25;10:337. doi: 10.1186/1471-2334-10-337. BMC Infect Dis. 2010. PMID: 21108793 Free PMC article.
Original investigations were eligible if they estimated sensitivity and specificity, or reliability -or if their calculation was possible - of ELISA or PCR tests, for chronic Chagas disease. RESULTS: Heterogeneity was high within each test (ELISA and PCR) and thresh …
Original investigations were eligible if they estimated sensitivity and specificity, or reliability -or if their calculation was possible - …
Enzyme-Linked Immunosorbent Assays.
Hornbeck PV. Hornbeck PV. Curr Protoc Immunol. 2015 Aug 3;110:2.1.1-2.1.23. doi: 10.1002/0471142735.im0201s110. Curr Protoc Immunol. 2015. PMID: 26237010
This unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell-surface antigens. ...One of the support protocols can be used to optimize the different ELISAs. A second support protocol presents a method for prepar …
This unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell-surface antigens. …
Mycotoxin Quantification by Competitive ELISA.
Merrill BE, Matson RS. Merrill BE, et al. Methods Mol Biol. 2023;2612:183-194. doi: 10.1007/978-1-0716-2903-1_14. Methods Mol Biol. 2023. PMID: 36795368
Enzyme-linked immunosorbent assays (ELISAs) are useful in the quantification of antigens like mycotoxins. ...The final corn and wheat samples were analyzed using a competitive ELISA specific to ZEA....
Enzyme-linked immunosorbent assays (ELISAs) are useful in the quantification of antigens like mycotoxins. ...The …
Methods of Cryptococcal Polysaccharide Analysis Using ELISA.
Wear MP, McConnell SA, Greengo SD, Lopes LL, Casadevall A. Wear MP, et al. Methods Mol Biol. 2024;2775:239-255. doi: 10.1007/978-1-0716-3722-7_16. Methods Mol Biol. 2024. PMID: 38758322
Here we present three variations of the GXM ELISA used by our group-indirect, capture, and competition ELISAs. We have also provided some history, perspective, and notes on these methods, which we hope will help the reader choose, and implement, the best assay …
Here we present three variations of the GXM ELISA used by our group-indirect, capture, and competition ELISAs. We have also pr …
Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers.
Lenz KD, Klosterman KE, Mukundan H, Kubicek-Sutherland JZ. Lenz KD, et al. Anal Biochem. 2022 Sep 1;652:114747. doi: 10.1016/j.ab.2022.114747. Epub 2022 May 27. Anal Biochem. 2022. PMID: 35636461 Free article.
Enzyme-linked immunosorbent assays (ELISAs) are widely employed for the detection of protein targets due to their ease of use, sensitivity, and potential for high-throughput analyses. ...An assay to detect the glycolipid lipoarabinomannan (LAM), …
Enzyme-linked immunosorbent assays (ELISAs) are widely employed for the detection of protein targets due to thei …
ELISA Methods for the Detection of Ebolavirus Infection.
Cross RW, Ksiazek TG. Cross RW, et al. Methods Mol Biol. 2017;1628:363-372. doi: 10.1007/978-1-4939-7116-9_29. Methods Mol Biol. 2017. PMID: 28573635
Here we describe methods for preparing antigen detection sandwich enzyme-linked immunosorbent assays (ELISAs) as well as IgG and IgM ELISAs for the detection of ebolavirus antigens or antibodies in biological samples....
Here we describe methods for preparing antigen detection sandwich enzyme-linked immunosorbent assays (ELISAs) as …
ELISA Evaluation of Tau Accumulation in the Brains of Patients with Alzheimer Disease.
Shinohara M, Hirokawa J, Shimodaira A, Tashiro Y, Suzuki K, Gheni G, Fukumori A, Matsubara T, Morishima M, Saito Y, Murayama S, Sato N. Shinohara M, et al. J Neuropathol Exp Neurol. 2021 Aug 11;80(7):652-662. doi: 10.1093/jnen/nlab047. J Neuropathol Exp Neurol. 2021. PMID: 34283221
Despite the routine use of sandwich enzyme-linked immunosorbent assays (ELISAs) for quantifying tau levels in CSF and plasma, tau accumulations in the brains of patients with Alzheimer disease (AD) have rarely been evaluated by this method. ...Moreover …
Despite the routine use of sandwich enzyme-linked immunosorbent assays (ELISAs) for quantifying tau levels in CS …
In-house ELISA protocols for capsid p24 detection of diverse HIV isolates.
Molina MA, Vink M, Berkhout B, Herrera-Carrillo E. Molina MA, et al. Virol J. 2023 Nov 17;20(1):269. doi: 10.1186/s12985-023-02242-5. Virol J. 2023. PMID: 37978551 Free PMC article.
BACKGROUND: The capsid p24 (CA-p24) antigen is a component of the viral capsid of human immunodeficiency virus (HIV) that has been commonly used for clinical diagnosis and monitoring of HIV infections in Enzyme-linked Immunosorbent Assays (ELISAs). Com …
BACKGROUND: The capsid p24 (CA-p24) antigen is a component of the viral capsid of human immunodeficiency virus (HIV) that has been commonly …
357,804 results
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