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354,497 results

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A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA.
Aydin S. Aydin S. Peptides. 2015 Oct;72:4-15. doi: 10.1016/j.peptides.2015.04.012. Epub 2015 Apr 20. Peptides. 2015. PMID: 25908411
These peptide/protein concentrations in biological fluids are measured using various methods, the most common of which is enzymatic immunoassay EIA/ELISA and which guide the clinicians in diagnosing and monitoring diseases that inflict biological systems. All the technique …
These peptide/protein concentrations in biological fluids are measured using various methods, the most common of which is enzymatic immunoas …
Direct ELISA.
Lin AV. Lin AV. Methods Mol Biol. 2015;1318:61-7. doi: 10.1007/978-1-4939-2742-5_6. Methods Mol Biol. 2015. PMID: 26160564
First described by Engvall and Perlmann, the enzyme-linked immunosorbent assay (ELISA) is a rapid and sensitive method for detection and quantitation of an antigen using an enzyme-labeled antibody. ...Depending on the antigen epitope and availab …
First described by Engvall and Perlmann, the enzyme-linked immunosorbent assay (ELISA) is a rapid and sen …
An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids.
Aydin S, Emre E, Ugur K, Aydin MA, Sahin İ, Cinar V, Akbulut T. Aydin S, et al. J Int Med Res. 2025 Feb;53(2):3000605251315913. doi: 10.1177/03000605251315913. J Int Med Res. 2025. PMID: 39922798 Free PMC article. Review.
The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. ...The article discusses the history of the assay, its underlying …
The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-label …
Enzyme-Linked Immunosorbent Assays.
Hornbeck PV. Hornbeck PV. Curr Protoc Immunol. 2015 Aug 3;110:2.1.1-2.1.23. doi: 10.1002/0471142735.im0201s110. Curr Protoc Immunol. 2015. PMID: 26237010
This unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell-surface antigens. ...One of the support protocols can be used to optimize the different ELISAs. A second support protocol presents a method for prepar …
This unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell-surface antigens. …
[ELISA for diagnosis of infections by viruses].
Yoshihara N. Yoshihara N. Nihon Rinsho. 1995 Sep;53(9):2277-82. Nihon Rinsho. 1995. PMID: 7474393 Review. Japanese.
HBV, HCV, HIV and HTLV, is the enzyme linked immunosorbent assay (ELISA), whose sensitivity and practicability have rendered it the most common primary screening assay. ELISA can be mass screening used automatic or semiautomatic ma …
HBV, HCV, HIV and HTLV, is the enzyme linked immunosorbent assay (ELISA), whose sensitivity and practicab …
ELISA versus PCR for diagnosis of chronic Chagas disease: systematic review and meta-analysis.
Brasil PE, De Castro L, Hasslocher-Moreno AM, Sangenis LH, Braga JU. Brasil PE, et al. BMC Infect Dis. 2010 Nov 25;10:337. doi: 10.1186/1471-2334-10-337. BMC Infect Dis. 2010. PMID: 21108793 Free PMC article.
Original investigations were eligible if they estimated sensitivity and specificity, or reliability -or if their calculation was possible - of ELISA or PCR tests, for chronic Chagas disease. RESULTS: Heterogeneity was high within each test (ELISA and PCR) and thresh …
Original investigations were eligible if they estimated sensitivity and specificity, or reliability -or if their calculation was possible - …
Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers.
Lenz KD, Klosterman KE, Mukundan H, Kubicek-Sutherland JZ. Lenz KD, et al. Anal Biochem. 2022 Sep 1;652:114747. doi: 10.1016/j.ab.2022.114747. Epub 2022 May 27. Anal Biochem. 2022. PMID: 35636461 Free article.
Enzyme-linked immunosorbent assays (ELISAs) are widely employed for the detection of protein targets due to their ease of use, sensitivity, and potential for high-throughput analyses. ...An assay to detect the glycolipid lipoarabinomannan (LAM),
Enzyme-linked immunosorbent assays (ELISAs) are widely employed for the detection of protein targets due to thei
In-house ELISA protocols for capsid p24 detection of diverse HIV isolates.
Molina MA, Vink M, Berkhout B, Herrera-Carrillo E. Molina MA, et al. Virol J. 2023 Nov 17;20(1):269. doi: 10.1186/s12985-023-02242-5. Virol J. 2023. PMID: 37978551 Free PMC article.
BACKGROUND: The capsid p24 (CA-p24) antigen is a component of the viral capsid of human immunodeficiency virus (HIV) that has been commonly used for clinical diagnosis and monitoring of HIV infections in Enzyme-linked Immunosorbent Assays (ELISAs). Com …
BACKGROUND: The capsid p24 (CA-p24) antigen is a component of the viral capsid of human immunodeficiency virus (HIV) that has been commonly …
Interval estimation for concentration in the ELISA setting.
Stephenson M, Bursa F, Ladroue C. Stephenson M, et al. J Immunol Methods. 2021 Oct;497:113122. doi: 10.1016/j.jim.2021.113122. Epub 2021 Aug 6. J Immunol Methods. 2021. PMID: 34364892
Enzyme-linked immunosorbent assays (ELISAs) are often used to quantify the concentration of biological substances. ...Here we compare profile likelihood interval estimation procedures to Wald type intervals for the interval estimation of a concentratio
Enzyme-linked immunosorbent assays (ELISAs) are often used to quantify the concentration of biological substance
A Unique Multiplex ELISA to Profile Growth Factors and Cytokines in Cerebrospinal Fluid.
Madiraju C, Sastry A, Oppong M, Karp J, Krajewska M, Krajewski S, Tomik B, Szczudlik A, Matson RS. Madiraju C, et al. Methods Mol Biol. 2023;2612:157-168. doi: 10.1007/978-1-0716-2903-1_12. Methods Mol Biol. 2023. PMID: 36795366
Multiplex arrays designed for enzyme-linked immunosorbent assays (ELISAs) are robust and cost-effective for profiling biomarkers. ...Results indicate that multiplex assay designed for the sandwich ELISA method is a unique, robust, and cos …
Multiplex arrays designed for enzyme-linked immunosorbent assays (ELISAs) are robust and cost-effective for prof …
354,497 results
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