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. 2009 Jun;15(6):928-31.
doi: 10.3201/eid1506.081610.

Diversity of Anaplasma phagocytophilum strains, USA

Eric Morissette  1 , Robert F Massung, Janet E Foley, A Rick Alleman, Patrick Foley, Anthony F Barbet
Affiliations

Diversity of Anaplasma phagocytophilum strains, USA

Eric Morissette et al. Emerg Infect Dis. 2009 Jun.

Abstract

We analyzed the structure of the expression site encoding the immunoprotective protein MSP2/P44 from multiple Anaplasma phagocytophilum strains in the United States. The sequence of p44ESup1 had diverged in Ap-variant 1 strains infecting ruminants. In contrast, no differences were detected between A. phagocytophilum strains infecting humans and domestic dogs.

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Figures

Figure 1
Figure 1
Diversity in the amino acid sequences encoded by p44ESup1/omp-1n in US and European strains of Anaplasma phagocytophilum. All strains are from the United States (the state is indicated in the strain designation) except for the strain from the sheep from Norway (SheepNor) and the dog from Sweden (DogSw). Human-origin strains are HZNY, NY18, NY37, WebWI, and HGE2MN; dog strains are Dog1MN, Dog2MN, and DogNY; wood rat (Neotoma fuscipes) strains are WR1CA and WR2CA; the horse strain is HorMRKCA; Ap-V1 strains are Ap1RIC (culture derived), Ap1RIG (isolated from an infected goat), Ap1MN29B, and Ap1MN62 (both Ap1MN strains were derived from naturally infected Ixodes scapularis ticks). Sequences were from either this study or GenBank: accession nos. DQ519565 (SheepNor), DQ519566 (DogSw), CP000235 (HZ), AY164490 (NY18), AY137510 (NY37), AY164491 (Webster), and AY164492 (HGE2).
Figure 2
Figure 2
Maximum-likelihood phylogram of different variants of Anaplasma phagocytophilum based on the p44ESup1/omp-1n and intergenic region gene sequences created by using TREEFINDER (www.treefinder.de) with default values. The number of substitutions per site over 1,092 total sites is shown under the tree, and bootstrap support for each split (percentage of times recovered) is shown next to each branch of the tree.
See this image and copyright information in PMC

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