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. 2010 Jun 5;401(2):190-6.
doi: 10.1016/j.virol.2010.01.033. Epub 2010 Mar 15.

Genetic characterization of HIV-1 from semen and blood from clade C-infected subjects from India and effect of therapy in these body compartments

Chengli Shen  1 , Ming Ding, Jodi K Craigo, Patrick Tarwater, Ramdas Chatterjee, Pratima Roy, Subhasish K Guha, Bibhuti Saha, Dolonchapa Modak, Dhrubak Neogi, Yue Chen, Phalguni Gupta
Affiliations

Genetic characterization of HIV-1 from semen and blood from clade C-infected subjects from India and effect of therapy in these body compartments

Chengli Shen et al. Virology. .

Abstract

Biologic and genetic differences between HIV-1 clade C in India and clade B in US suggest that the effect of anti-viral therapy in various body compartments may differ between these two clades. We examined the effect of therapy on viral loads in semen and blood of HIV-1-clade C infected subjects from India and evaluated whether HIV-1 in the semen is different from that in blood in these subjects. HIV-1 RNA was detected in semen and blood at all stages of the disease. Viral loads in semen and blood were strongly correlated with each other, but not with the CD4+ T cell count. Anti-viral treatment reduced viral load drastically in blood and semen within one month of post therapy. Genetic characterization of HIV-1 in the semen and blood demonstrated that they were highly compartmentalized. These data have important implications of sexual transmission of HIV-1 in clade C HIV-1 infected subjects.

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Figures

Figure 1
Figure 1. Viral load of bloods plasma and seminal plasma before and after potent antiretroviral therapy
Figure 2
Figure 2. Diversity and distance analysis of envelope sequences (C2–V5) of HIV-1 from Indian patients using PAUP* software
(A) Nucleic acid diversity of corrected intrapopulation diversity was calculated for nucleotide sequences. The quartile (box) differences between the diversity of all sequence clones were plotted as interpopulation distances as a function of different compartments. (B) Corrected population distance between blood derived plasma and cells and semen derived plasma and cells was calculated for nucleotide sequences. Statistical significance of the differences between different compartments for each analysis was performed using nonparametric statistics, the Mann-Whitney test. BP, blood plasma; BC, blood cells; SP, semen plasma; SC, semen cells. Blood compartment, distance between blood plasma and blood cells; Semen compartment, distance between seminal plasma and seminal cells.
Figure 3
Figure 3. Immune selection analysis of subgenome of C2-V5
Analysis of immune selection at various subgenomic regions (C2, V3, C3, V4, C4, and V5) of the whole C2-V5 sequences from four body compartments (seminal cells and plasma, and blood cells and plasma).
See this image and copyright information in PMC

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