Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 May;47(5):1463-8.
doi: 10.1128/JCM.02467-08. Epub 2009 Mar 25.

Less-frequent Fusarium species of clinical interest: correlation between morphological and molecular identification and antifungal susceptibility

Mónica Azor  1 , Josepa Gené, Josep Cano, Palanisamy Manikandan, Narendran Venkatapathy, Josep Guarro
Affiliations

Less-frequent Fusarium species of clinical interest: correlation between morphological and molecular identification and antifungal susceptibility

Mónica Azor et al. J Clin Microbiol. 2009 May.

Abstract

Forty-eight Fusarium isolates morphologically identified as belonging to seven species of clinical interest (i.e., Fusarium chlamydosporum, Fusarium dimerum, Fusarium incarnatum, Fusarium napiforme, Fusarium nygamai, Fusarium proliferatum, and Fusarium sacchari) were characterized molecularly by the analysis of the sequences of the TUB region of the beta-tubulin gene. F. chlamydosporum and F. dimerum were the most genetically heterogeneous species. A high degree of correlation between the morphological and molecular identification was shown among the isolates studied. A table with the key morphological features for the identification of these Fusarium species is provided. The antifungal susceptibilities of the Fusarium isolates to 11 antifungal drugs were tested; terbinafine was the most active drug against all the species tested with the exception of F. incarnatum, for which amphotericin B was the most active.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
One of the 225 most parsimonious trees obtained from heuristic searches based on TUB sequences. Bootstrap support values are indicated at the nodes. CI, consistency index; HI, homoplasy index; RI, retention index; asterisks, accession numbers of sequences retrieved from GenBank.
See this image and copyright information in PMC

References

    1. Alastruey-Izquierdo, A., M. Cuenca-Estrella, A. Monzón, E. Mellado, and J. L. Rodríguez-Tudela. 2008. Antifungal susceptibility profile of clinical Fusarium spp. isolates identified by molecular methods. J. Antimicrob. Chemother. 61805-809. - PubMed
    1. Austen, B., H. McCarthy, B. Wilkins, A. Smith, and A. Duncombe. 2001. Fatal disseminated Fusarium infection in acute lymphoblastic leukaemia in complete remission. J. Clin. Pathol. 54488-490. - PMC - PubMed
    1. Azor, M., J. Gené, J. Cano, and J. Guarro. 2007. Universal in vitro antifungal resistance of genetic clades of the Fusarium solani species complex. Antimicrob. Agents Chemother. 511500-1503. - PMC - PubMed
    1. Azor, M., J. Gené, J. Cano, D. A. Sutton, A. W. Fothergill, M. G. Rinaldi, and J. Guarro. 2008. In vitro antifungal susceptibility and molecular characterization of clinical isolates of Fusarium verticillioides (F. moniliforme) and Fusarium thapsinum. Antimicrob. Agents Chemother. 522228-2231. - PMC - PubMed
    1. Balajee, S. A., A. M. Borman, M. E. Brandt, J. Cano, M. Cuenca-Estrella, E. Dannaoui, J. Guarro, G. Haase, C. C. Kibbler, W. Meyer, K. O'Donnell, C. A. Petti, J. L. Rodriguez-Tudela, D. Sutton, A. Velegraki, and B. L. Wickes. 2009. Sequence-based identification of Aspergillus, Fusarium, and Mucorales in the clinical mycology laboratory: where are we and where should we go from here? J. Clin. Microbiol. 47877-884. - PMC - PubMed

Publication types

Associated data