Structural basis for selective binding of m6A RNA by the YTHDC1 YTH domain

Chao Xu¹, Xiao Wang², Ke Liu³, Ian A Roundtree⁴, Wolfram Tempel⁵, Yanjun Li⁵, Zhike Lu⁶, Chuan He⁶, Jinrong Min⁷

Affiliations

¹ 1] Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada. [2]. chaor.xu@utoronto.edu.
² 1] Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA. [2] Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA. [3].
³ 1] Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada. [2].
⁴ 1] Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA. [2] Medical Scientist Training Program and Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
⁵ Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada.
⁶ 1] Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA. [2] Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA.
⁷ 1] Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada. [2] Department of Physiology, University of Toronto, Toronto, Ontario, Canada.

PMID: 25242552
DOI: 10.1038/nchembio.1654

Structural basis for selective binding of m6A RNA by the YTHDC1 YTH domain

Chao Xu et al. Nat Chem Biol. 2014 Nov.

. 2014 Nov;10(11):927-9.

doi: 10.1038/nchembio.1654. Epub 2014 Sep 21.

Authors

Chao Xu¹, Xiao Wang², Ke Liu³, Ian A Roundtree⁴, Wolfram Tempel⁵, Yanjun Li⁵, Zhike Lu⁶, Chuan He⁶, Jinrong Min⁷

Affiliations

¹ 1] Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada. [2]. chaor.xu@utoronto.edu.
² 1] Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA. [2] Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA. [3].
³ 1] Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada. [2].
⁴ 1] Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA. [2] Medical Scientist Training Program and Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
⁵ Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada.
⁶ 1] Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA. [2] Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA.
⁷ 1] Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada. [2] Department of Physiology, University of Toronto, Toronto, Ontario, Canada.

PMID: 25242552
DOI: 10.1038/nchembio.1654

Erratum in

Corrigendum: Structural basis for selective binding of m(6)A RNA by the YTHDC1 YTH domain.
Xu C, Wang X, Liu K, Roundtree IA, Tempel W, Li Y, Lu Z, He C, Min J. Xu C, et al. Nat Chem Biol. 2015 Oct;11(10):815. doi: 10.1038/nchembio1015-815c. Nat Chem Biol. 2015. PMID: 26379027 No abstract available.

Abstract

N(6)-methyladenosine (m(6)A) is the most abundant internal modification of nearly all eukaryotic mRNAs and has recently been reported to be recognized by the YTH domain family proteins. Here we present the crystal structures of the YTH domain of YTHDC1, a member of the YTH domain family, and its complex with an m(6)A-containing RNA. Our structural studies, together with transcriptome-wide identification of YTHDC1-binding sites and biochemical experiments, not only reveal the specific mode of m(6)A-YTH binding but also explain the preferential recognition of the GG(m(6)A)C sequences by YTHDC1.

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References

1. J Biol Chem. 2010 May 7;285(19):14701-10 - PubMed
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Structural basis for selective binding of m6A RNA by the YTHDC1 YTH domain

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Structural basis for selective binding of m6A RNA by the YTHDC1 YTH domain

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