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. 2010 Jun;54(6):2724-7.
doi: 10.1128/AAC.01674-09. Epub 2010 Apr 12.

OXA-24 carbapenemase gene flanked by XerC/XerD-like recombination sites in different plasmids from different Acinetobacter species isolated during a nosocomial outbreak

María Merino  1 , Joshi Acosta, Margarita Poza, Francisca Sanz, Alejandro Beceiro, Fernando Chaves, Germán Bou
Affiliations

OXA-24 carbapenemase gene flanked by XerC/XerD-like recombination sites in different plasmids from different Acinetobacter species isolated during a nosocomial outbreak

María Merino et al. Antimicrob Agents Chemother. 2010 Jun.

Abstract

A clinical strain of Acinetobacter calcoaceticus resistant to carbapenems was isolated from a blood culture sample from an inpatient in a hospital in Madrid (Spain) during a large outbreak of infection (affecting more than 300 inpatients), caused by a multidrug-resistant Acinetobacter baumannii clone. The carbapenem resistance in both the A. calcoaceticus and A. baumannii clones was due to a bla(OXA-24) gene harbored in different plasmids. The plasmids were fully sequenced, revealing the presence of site-specific recombination binding sites putatively involved in mobilization of the bla(OXA-24) gene. Comparison of plasmids contained in the two strains revealed possible horizontal transmission of resistance genes between the Acinetobacter species.

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Figures

FIG. 1.
FIG. 1.
(Top) Diagram of the 8,771-bp pMMCU1 plasmid obtained from the A. calcoaceticus clinical strain (Acal H12O-07). (Bottom) Diagram of the 10,679-bp pMMA2 plasmid obtained from the A. baumannii strain (AbH12O-A2) that caused the large outbreak of infection. The asterisks indicate the Xer-like recombination sites.
See this image and copyright information in PMC

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