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. 2010 Dec;48(12):4636-40.
doi: 10.1128/JCM.01090-10. Epub 2010 Oct 13.

Identification of a novel t17 genotype of acanthamoeba from environmental isolates and t10 genotype causing keratitis in Thailand

Warisa Nuprasert  1 , Chaturong Putaporntip, Lalida Pariyakanok, Somchai Jongwutiwes
Affiliations

Identification of a novel t17 genotype of acanthamoeba from environmental isolates and t10 genotype causing keratitis in Thailand

Warisa Nuprasert et al. J Clin Microbiol. 2010 Dec.

Abstract

We analyzed the nuclear small-subunit rRNA genes of Acanthamoeba isolates from freshwater sources (n=16) and from patients (n=6) in Bangkok and surrounding areas. The T10 genotype from a keratitis patient and a novel T17 genotype from water samples were diagnosed for the first time in this study.

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Figures

FIG. 1.
FIG. 1.
The Rns genealogy of Acanthamoeba isolates from Thailand inferred by the neighbor-joining method using the maximum composite likelihood model. Bootstrap values ≥70% from 1,000 pseudoreplicates are shown next to the branches. The GenBank accession numbers of known T1 to T16 genotypes are in parentheses. Filled circles and filled diamonds represent clinical isolates and genotype “T17,” respectively.
FIG. 2.
FIG. 2.
Photomicrograph of Acanthamoeba genotype “T17” depicting trophozoites of isolates ACE1 (A) and ACE9 (C) and their respective cysts (B and D). Bars, 10 μm.
See this image and copyright information in PMC

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