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. 2011 Aug;5(8):1237-41.
doi: 10.1038/ismej.2011.10. Epub 2011 Mar 10.

Accessing marine protists from the anoxic Cariaco Basin

V Edgcomb  1 , W Orsi, G T Taylor, P Vdacny, C Taylor, P Suarez, S Epstein
Affiliations

Accessing marine protists from the anoxic Cariaco Basin

V Edgcomb et al. ISME J. 2011 Aug.
No abstract available

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Figures

Figure 1
Figure 1
Scanning electron micrographs of protists from the Cariaco Basin's anoxic, sulfidic, deepwaters (900 m). (a–d) Flagellates; (g–i) possible scuticociliates with (e, f) epibiotic bacteria. Scale bars: 2 μm (c, e, f), 5 μm (b, d, h, i), 10 μm (g) and 20 μm (a). (j) The number of protistan cells visualized by FISH with a ‘universal' eukaryotic probe (solid line) versus their total number determined by SEM (dotted line). A: 40 m above the oxic/anoxic interface, I: interface (approximately 250 m depth), B: 40 m below the interface, D: 900 m. B, epibiotic bacteria; CC, caudal cilia; F, flagellum; FU, furrow; PM, paroral membrane; PS, pseudopodia; SK, somatic cilia; V, vestibulum.
Figure 2
Figure 2
Phylogenetic relationships of ciliate 18S rRNA gene sequences obtained by the cDNA approach, as well as direct amplification of the gene from environmental samples. Bootstrap (RAxML) and posterior probability values greater than 50% are shown at the nodes in the order PP/ML. Black circles at the nodes represent full posterior probability and bootstrap support. Centroid sequences from our 18S rRNA gene survey (see Edgcomb et al. (2011) and Orsi et al. (2011)) are in bold font. All cDNA sequences fell into the ‘cDNA clade'. The number of cDNA sequences and those from our 18S rRNA gene survey are shown in the collapsed triangle (30 cDNA sequences, 2718S rRNA gene sequences).
See this image and copyright information in PMC

References

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