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. 2011 Jan;49(1):419-22.
doi: 10.1128/JCM.01871-10. Epub 2010 Nov 17.

Novel multiplex oligonucleotide-conjugated bead suspension array for rapid identification of enterovirus 71 subgenogroups

Y Wu  1 , E L Tan, A Yeo, K P Chan, H Nishimura, M J Cardosa, C L Poh, S H Quak, Vincent T Chow
Affiliations

Novel multiplex oligonucleotide-conjugated bead suspension array for rapid identification of enterovirus 71 subgenogroups

Y Wu et al. J Clin Microbiol. 2011 Jan.

Abstract

A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successfully detected and correctly subgenogrouped.

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Figures

FIG. 1.
FIG. 1.
Schematic representation of the multiplex suspension array system for EV71 subgenogrouping. The VP1 region was amplified by consensus primers, and the RT-PCR product was purified by ExoSAP-IT. ASPE was performed, and only perfectly complementary strands would be amplified. The ASPE products were labeled using biotin-dCTP (black stars), and hybridized to their complementary anti-tag oligonucleotide sequences on the beads or microspheres. The complex was subjected to the Luminex reader and was identified by both lasers and streptavidin-phycoerythrin (SAPE).
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References

    1. Baskaran, N., et al. 1996. Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content. Genome Res. 6:633-638. - PubMed
    1. Brown, B. A., M. S. Oberste, J. P. Alexander, M. L. Kennett, and M. A. Pallansch. 1999. Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998. J. Virol. 73:9969-9975. - PMC - PubMed
    1. Cardosa, M. J., et al. 2003. Molecular epidemiology of human enterovirus 71 strains and recent outbreaks in the Asia-Pacific region: comparative analysis of the VP1 and VP4 genes. Emerg. Infect. Dis. 9:461-468. - PMC - PubMed
    1. Chan, K. P., et al. 2003. Epidemic hand, foot and mouth disease caused by human enterovirus 71, Singapore. Emerg. Infect. Dis. 9:78-85. - PMC - PubMed
    1. Ginocchio, C. C., and K. St. George. 2009. Likelihood that an unsubtypeable influenza A virus result obtained with the Luminex xTAG respiratory virus panel is indicative of infection with novel A/H1N1 (swine-like) influenza virus. J. Clin. Microbiol. 47:2347-2348. - PMC - PubMed

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