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. 1989 Mar;171(3):1372-8.
doi: 10.1128/jb.171.3.1372-1378.1989.

Cloning, purification, and properties of Candida albicans thymidylate synthase

S C Singer  1 , C A Richards, R Ferone, D Benedict, P Ray
Affiliations

Cloning, purification, and properties of Candida albicans thymidylate synthase

S C Singer et al. J Bacteriol. 1989 Mar.

Abstract

The thymidylate synthase (TS) gene was isolated from a genomic Candida albicans library by functional complementation of a Saccharomyces cerevisiae strain deficient in TS. The gene was localized on a 4-kilobase HindIII DNA fragment and was shown to be expressed in a Thy- strain of Escherichia coli. The nucleotide sequence of the TS gene predicted a protein of 315 amino acids with a molecular weight of 36,027. The gene was cloned into a T7 expression vector in E. coli, allowing purification of large amounts of C. albicans TS. It was also purified from a wild-type C. albicans strain. Comparison of several enzyme properties including analysis of amino-terminal amino acid sequences showed the native and cloned C. albicans TS to be the same.

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