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. 2012 Aug;87(2):349-58.
doi: 10.4269/ajtmh.2012.11-0666.

Molecular characterization and phylogenetic analysis of dengue viruses imported into Taiwan during 2008-2010

Jyh-Hsiung Huang  1 , Chien-Ling Su, Cheng-Fen Yang, Tsai-Ling Liao, Tung-Chien Hsu, Shu-Fen Chang, Chien-Chou Lin, Pei-Yun Shu
Affiliations

Molecular characterization and phylogenetic analysis of dengue viruses imported into Taiwan during 2008-2010

Jyh-Hsiung Huang et al. Am J Trop Med Hyg. 2012 Aug.

Abstract

We present our surveillance results on imported dengue cases in Taiwan during 2008-2010. A total of 734 imported dengue patients were identified. The travelers were arriving from 18 countries, including Southeast Asia, the Indian subcontinent, South Pacific islands, and Latin America. Gene sequences from 358 dengue virus (DENV) isolates were used to perform phylogenetic analyses, thus, providing an updated view of the geographic distribution and dynamic transmission of DENV strains circulating in these countries. Our results suggest that the DENV-1 genotype I and DENV-2 Cosmopolitan genotype comprise the predominant DENV strains circulating in Southeast Asian countries. The DENV-3 Genotype III strain was found to be newly emerging in several Southeast Asian countries, however, the Asian genotype 2 and the Asian/American genotype of DENV-2 strains appeared to be less prevalent in Southeast Asia. Furthermore, imported dengue viruses are representative of the overall patterns of serotype/genotype frequencies of dengue outbreaks that occurred in Taiwan.

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Figures

Figure 1.
Figure 1.
A phylogenetic tree of dengue virus type 1 (DENV-1). The phylogenetic tree is based on the complete E-gene sequences of 85 strains of DENV-1 including 57 Taiwan isolates from imported and indigenous dengue cases during 2008–2010. The tree was constructed by the maximum likelihood method and the general time-reversible model. Bootstrap support values greater than 75 are shown. The Taiwan isolates from imported cases during 2008–2010 are designated in boldface. The Taiwan isolates from indigenous cases are designated in boldface and solid triangle (▲). Viruses were identified by using the nomenclature of serotype/country/strain/year of isolation/GenBank accession number. The scale bar on the left indicates substitutions per site.
Figure 2.
Figure 2.
A phylogenetic tree of dengue virus type 2 (DENV-2). The phylogenetic tree is based on the complete E-gene sequences of 75 strains of DENV-2 including 54 Taiwan isolates from imported and indigenous dengue cases during 2008–2010. See the legend of Figure 1 for other details.
Figure 3.
Figure 3.
A phylogenetic tree of dengue virus type 3 (DENV-3). The phylogenetic tree is based on the complete E-gene sequences of 63 strains of DENV-3 including 38 Taiwan isolates from imported and indigenous dengue cases during 2008–2010. See the legend of Figure 1 for other details.
Figure 4.
Figure 4.
A phylogenetic tree of dengue virus type 4 (DENV-4). The phylogenetic tree is based on the complete E-gene sequences of 44 strains of DENV-4 including 23 Taiwan isolates from imported and indigenous dengue cases during 2008–2010. See the legend of Figure 1 for other details.
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