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. 2014 Jun;60(2):147-53.
doi: 10.1016/j.jcv.2014.02.005. Epub 2014 Feb 25.

Virus and cystic fibrosis: rhinoviruses are associated with exacerbations in adult patients

Anne Goffard  1 , Valérie Lambert  2 , Julia Salleron  3 , Stéphanie Herwegh  2 , Ilka Engelmann  4 , Claudine Pinel  5 , Isabelle Pin  6 , Thierry Perrez  7 , Anne Prévotat  7 , Anny Dewilde  2 , Laurence Delhaes  8
Affiliations

Virus and cystic fibrosis: rhinoviruses are associated with exacerbations in adult patients

Anne Goffard et al. J Clin Virol. 2014 Jun.

Abstract

Background: Few studies have suggested the potential role of respiratory viruses in cystic fibrosis (CF) exacerbation, but their real impact is probably underestimated.

Method: Sixty-four sputum samples collected from 46 adult patients were included in the study: 33 samples were collected during exacerbation of CF, and 31 during the stable phase. After extraction, nucleic acids were tested for the presence of respiratory viruses. When rhinovirus (HRV) was detected, the 5'UTR and VP4/2 regions were sequenced, and phylogenetically analyzed. The characteristics of patients in exacerbation and stable phase were compared.

Results: Viruses were found in 25% of samples. The HRV viruses were the most frequently detected followed by coronaviruses. Only the HRV detection was significantly associated with the occurrence of CF pulmonary exacerbation (p<0.027). Characterization of 5'UTR and VP4/2 regions of the HRV genome specified that HRV-A, -B, -C were detected. All HRV-C were recombinant HRV-Ca.

Conclusions: HRV were the most frequently detected viruses; their detection was significantly associated with the occurrence of an exacerbation. The reality of viral recombination between HRV was demonstrated in CF patients for the first time, raising the role of viruses in lung microbiota. Further studies are now warranted to decipher virus impact in CF.

Keywords: Cystic fibrosis; PCR; Pulmonary exacerbation; Rhinovirus.

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Figures

Fig. 2
Fig. 2
Phylogenetic trees of VP4/VP2 gene (A) and 5′UTR region (B) of HRVs. Trees were constructed from alignments of available sequences of complete (white circles), partial genomes (black circles) and sequences obtained from HRV-positive samples (gray triangles). HRV sequences were deposited in GenBank under the accession numbers KC492746–62.
Fig. 1
Fig. 1
Frequency and seasonal distribution of respiratory viruses detected from sputa of CF patients. In each subgroup composed of sputa collected during pulmonary exacerbation (A) or of sputa collected during stable period (B), positive and negative samples are presented as percentages from the total in each subgroup. Seasonal distribution (C) of HRV-A, dotted black line with diamond; of HRV-C, black line with square; HCoV-229E/NL63, gray line with diamond; and of HCoV-OC-43/HKU1, dotted gray line with square.
See this image and copyright information in PMC

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