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Comparative Study
. 1993 Apr 29;1173(1):107-10.
doi: 10.1016/0167-4781(93)90254-b.

Two developmentally regulated mRNAs encoding actin-binding proteins in Physarum polycephalum

B St-Pierre  1 , C Couture, A Laroche, D Pallotta
Affiliations
Comparative Study

Two developmentally regulated mRNAs encoding actin-binding proteins in Physarum polycephalum

B St-Pierre et al. Biochim Biophys Acta. .

Abstract

A cDNA library from amoebae of Physarum polycephalum was screened by differential hybridization. Two clones contained inserts for mRNAs present in amoebae and absent in plasmodia. The LAV3-4 cDNA encodes a 402 aa protein (ABP-46) that shows sequence similarity to the actin binding site in the N-terminal region of the alpha-actinin family. The LAV3-5 cDNA is 76% identical to the Dictyostelium actin bundling protein, which cross-links and stabilizes actin filaments in amoebal filopodia.

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