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Comparative Study
. 1993 Aug 31;195(1):53-7.
doi: 10.1006/bbrc.1993.2008.

Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum)

K C Ehrlich  1 , B G Montalbano, E J Mullaney, H C Dischinger Jr, A H Ullah
Affiliations
Comparative Study

Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum)

K C Ehrlich et al. Biochem Biophys Res Commun. .

Abstract

An Aspergillus niger (ficuum) genomic DNA lambda EMBL3 library was probed with a 354-bp DNA fragment obtained by polymerase chain reaction of A. niger DNA with oligonucleotides based on partial amino acid sequence of a pH 2.5 optimum acid phosphatase. A clone containing a 1605 bp segment (phyB) encoding the 479 amino acid enzyme was isolated and found to contain four exons. Global alignment revealed 23.5% homology to Aspergillus niger phytase (PhyA); four regions of extensive homology were identified. Some of these regions may contain catalytic sites for phosphatase function.

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