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. 1997 Apr 15;149(2):189-94.
doi: 10.1111/j.1574-6968.1997.tb10327.x.

Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon

A Brans  1 , A Loriaux, I Thamm, B Joris, J Dusart
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Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon

A Brans et al. FEMS Microbiol Lett. .

Abstract

The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.

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