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. 1978 Oct 19;275(5681):617-24.
doi: 10.1038/275617a0.

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

A C Chang, J H Nunberg, R J Kaufman, H A Erlich, R T Schimke, S N Cohen

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

A C Chang et al. Nature. .

Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.

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