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. 1986;47(2-3):179-83.
doi: 10.1016/0378-1119(86)90061-2.

Expression of synthetic suppressor tRNA genes under the control of a synthetic promoter

J M Masson, J H Miller

Expression of synthetic suppressor tRNA genes under the control of a synthetic promoter

J M Masson et al. Gene. 1986.

Abstract

A synthetic promoter derived from the Escherichia coli lipoprotein promoter sequence was used to express synthetic suppressor tRNA genes in E. coli. These constructs, cloned into a plasmid of the pEMBL family, gave very high yields of suppressor tRNA in vivo.

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