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. 1987 Jul;169(7):3082-7.
doi: 10.1128/jb.169.7.3082-3087.1987.

Translational fusion with a secretory enzyme as an indicator

P Z Wang, S J Projan, K R Leason, R P Novick

Translational fusion with a secretory enzyme as an indicator

P Z Wang et al. J Bacteriol. 1987 Jul.

Abstract

A novel type of translational fusion system has been developed by using a secretory protein, staphylococcal beta-lactamase, as an indicator. The beta-lactamase structural gene was modified to provide N-terminal extensions of 13 and 162 amino acids, and in both cases, the fusion protein was processed and the mature active enzyme was secreted; thus, the expression of a particular upstream gene can be analyzed by monitoring the beta-lactamase activity.

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References

    1. Nature. 1984 Jun 14-20;309(5969):637-9 - PubMed
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    1. EMBO J. 1984 Oct;3(10):2407-14 - PubMed
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