Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Jun 19;49(6):753-61.
doi: 10.1016/0092-8674(87)90613-1.

A trans-spliced leader sequence on actin mRNA in C. elegans

M Krause, D Hirsh

A trans-spliced leader sequence on actin mRNA in C. elegans

M Krause et al. Cell. .

Abstract

While determining the 5' ends of C. elegans actin mRNAs, we have discovered a 22 nucleotide spliced leader sequence. The leader sequence is found on mRNA from three of the four nematode actin genes. The leader also appears to be present on some, but not all, nonactin mRNAs. The actin mRNA leader sequence is identical to the first 22 nucleotides of a novel 100 nucleotide RNA transcribed adjacent, and in the opposite orientation, to the 5S ribosomal gene. The evidence suggests that the actin mRNA leader sequence is acquired from this novel nucleotide transcript by an intermolecular trans-splicing mechanism.

PubMed Disclaimer

References

    1. Albertson D.G. Localization of ribosomal genes in C. elegans chromosomes by in situ hybridization using biotin-labeled probes. EMBO J. 1984;3:1227. - PMC - PubMed
    1. Albertson D.G. Mapping muscle protein genes by in situ hybridization using biotin-labeled probes. EMBO J. 1985;4:2493–2498. - PMC - PubMed
    1. Birnstiel M.L., Busslinger M., Strub K. Transcription termination and 3′ end processing: the end is in site! Cell. 1985;4:349–359. - PubMed
    1. Campbell D.A., Thornton D.A., Boothroyd J.C. Apparent discontinuous transcription of Trypanosoma brucei variant surface antigen genes. Nature. 1984;311:350–355. - PMC - PubMed
    1. Cox G.N., Hirsh D. Stage-specific patterns of collagen gene expression during development of Caenorhabditis elegans. Mol. Cell. Biol. 1985;5:363–372. - PMC - PubMed

Publication types

Associated data

LinkOut - more resources