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. 1988 Apr;62(4):1180-5.
doi: 10.1128/JVI.62.4.1180-1185.1988.

Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase

L Mindich  1 , I Nemhauser, P Gottlieb, M Romantschuk, J Carton, S Frucht, J Strassman, D H Bamford, N Kalkkinen
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Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase

L Mindich et al. J Virol. 1988 Apr.

Abstract

The genome of the lipid-containing bacteriophage phi 6 contains three segments of double-stranded RNA. We determined the nucleotide sequence of cDNA derived from the largest RNA segment (L). This segment specifies the procapsid proteins necessary for transcription and replication of the phi 6 genome. The coding sequences of the four proteins on this segment were identified on the basis of size and the correlation of predicted N-terminal amino acid sequences with those found through analysis of isolated proteins. This report completes the sequence analysis of phi 6. This constitutes the first complete sequence of a double-stranded RNA genome virus.

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