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. 1995 Oct 20;270(5235):484-7.
doi: 10.1126/science.270.5235.484.

Serial analysis of gene expression

V E Velculescu  1 , L Zhang, B Vogelstein, K W Kinzler
Affiliations

Serial analysis of gene expression

V E Velculescu et al. Science. .

Abstract

The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.

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  • Entering the postgenome era.
    Nowak R. Nowak R. Science. 1995 Oct 20;270(5235):368-9, 371. doi: 10.1126/science.270.5235.368. Science. 1995. PMID: 7569988 No abstract available.

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