Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Apr 30;168(2):801-8.
doi: 10.1016/0006-291x(90)92392-d.

Cloning and expression of pectin lyase gene from Erwinia carotovora in Escherichia coli

T Nishida  1 , T Suzuki, K Ito, Y Kamio, K Izaki
Affiliations

Cloning and expression of pectin lyase gene from Erwinia carotovora in Escherichia coli

T Nishida et al. Biochem Biophys Res Commun. .

Abstract

A pectin lyase (PNL; EC 4.2.2.10) gene of Erwinia carotovora Er was cloned and expressed in Escherichia coli. The analysis of the nucleotide sequence of the 0.6 kb StuI-EcoRI fragment, which was hybridized with the mixed oligonucleotide probe for PNL gene, revealed the presence of an open reading frame (0RF) and correlated exactly with the known N-terminal 18 amino acid sequence of PNL. When a plasmid pTN2159, which has a BamHI-EcoRI fragment containing this ORF, was introduced into E. coli JM109, PNL was not expressed. When a tac-promoter was inserted in front of the ORF, PNL was efficiently expressed in E. coli. Synthesis of PNL by E. coli was also confirmed by immunoblot analysis.

PubMed Disclaimer

Associated data

LinkOut - more resources